Single Cell Cloning of Leishmania Parasites in Purine-Defined Medium: Isolation of Drug-Resistant Variants

  • David M. Iovannisci
  • Buddy Ullman
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 165)


A simple technique for the isolation of Leishmania donovani and Leishmania tropica promastigote clones derived from a single cell involves the use of semi-solid agar. Both species of Leishmania form discrete colonies at high efficiency in completely defined medium lacking serum. Visible colonies appear between eight and fourteen days. Viability of colonies transferred from semi-solid agar to liquid suspension culture is 100%. Using these techniques, we have isolated clonal populations of cells resistant to tubercidin and formycin b.


Cloning Efficiency Leishmania Species Leishmania Parasite Single Cell Cloning Visible Coloni 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Berens, R. L. and Marr, J.J. 1978. An easily prepared defined medium for cultivation of Leishmania donovani promastigotes. J. Parasitol. 64: 160.PubMedCrossRefGoogle Scholar
  2. Castellani, O., Ribeiro, L. V. and Fernandes, J. F. 1967. Differentiation of Typanosoma cruzi in culture. J. Parasitol. 14: 447–451.Google Scholar
  3. Iovannisci, D. M., and Ullman, B. 1982. A simple defined medium for the continuous cultivation of Leishmania promastigotes. Manuscript in preparation.Google Scholar
  4. Keppel, A. D. and Janovy, J., JR. 1980. Morphology of Leishmania donovani colonies grown on blood agar plates. J. Parasitol. 66 (5): 849–851.PubMedCrossRefGoogle Scholar
  5. Mattei, D. M., Goldberg, S., Morel, C., Azevedo, H.P., and Roitman, I. 1977. Biochemical strain characterization of Trypanosoma cruzi by restriction endonuclease cleavage of kinetoplast DNA. Federation of European Biochemical Societies Letters. 74: 264–268.PubMedCrossRefGoogle Scholar
  6. Steiger, R. E. and Steiger, E.. 1976. A defined medium for cultivating Leishmania donovani and L. braziliensis. J. Parasitol. 62: 1010–1011.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • David M. Iovannisci
    • 1
  • Buddy Ullman
    • 1
  1. 1.University of KentuckyLexingtonUSA

Personalised recommendations