Lymphocyte Ecto 5′-Nucleotidase in Immunodeficiency and Leukaemia
Circulating blood lymphocytes have relatively high activity of ecto 5′-nucleotidase (EC 22.214.171.124-5′N) as compared to monocytes of neutrophils (Webster et al 1978). This activity varies between different subpopulations; B lymphocytes having about four times the activity of ‘E’ rosetting T lymphocytes (Rowe et al 1980). 5′N activity also varies between T cell subpopulations, OKT8 (suppressor) T cells having about half the activity of OKT4 (helper) cells (Boss et al 1980, Massaia et al 1982). Pro-thymocytes and cortical thymocytes have very low 5′N activity, medullary thymocytes and cord blood T cells having about half the activity of circulating T cells (Fig.1). The 5′-N activity in cortical thymocytes can be increased by incubation with either thymic epithelial cells or thymosin (Cohen et al (1981).
KeywordsSevere Combine Immunodeficiency Purine Metabolism Thymic Epithelial Cell Nucleotidase Activity Obligate Heterozygote
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- Johnson, S.M., North, M.E., Asherson, G.L., Allsop, J., Watts, R.W.E. and Webster, A.D.B., 1977, Lymphocyte purine 5’-nucleotidase deficiency in primary hypogammaglobulinaemia, Lancet i: 168–170.Google Scholar
- Levin, A., Jones, M., Shah, T. and Peters, T.J., 1982, unpublished. Ma, D.D.F., Sylwestrowicz, T.A., Massaia, M., Price, G., Tidman, N. and Hoffbrand, A.V., 1982, unpublished.Google Scholar
- Massaia, M., Ma, D.D.F., Sylwestrowicz, T.A., Tidman, N., Price, G. and Hoffbrand, A.V., 1982, Enzymes of purine metabolism in human peripheral lymphocyte populations, Clin.Exp.Immunol. (in press).Google Scholar
- Matamoros, N., Abad, E. and Webster, A.D.B., 1982, LDH isoenzymes in the T lymphocytes of patients with primary hypogammaglobulinaemia, Clin.Exp.Immunol., in press.Google Scholar
- Rowe, M., DeGast, C.G., Platts-Mills, T.A.E., Asherson, G.L., Webster, A.D.B. and Johnson, S.M., 1979, 5’-nucleotidase of B and T lymphocytes isolated from human peripheral blood, Clin. Exp. Immunol., 36: 97–101.Google Scholar
- Shah, T., Webster, A.D.B., Peters, T.J., 1982, unpublished.Google Scholar
- Sylwestrowicz, T., Piga, A., Murphy, P., Ganeshaguru, K., Russell, N.H., Prentice, H.G. and Hoffbrand, A.V., 1982, The effects of deoxycoformycin and deoxyadenosine on deoxyribonucleotide concentrations in leukaemia cells, Brit.J.Haemat., 51.Google Scholar
- Thompson, L.F., Boss, G.R., Spiegelberg, H.L., Jansen, I.V., O’Connor, R.D., Waldmann, T.A., Bamberger, R.N. and Seegmiller, J.E., 1979, Ecto-5’-nucleotidase activity in T and B lymphocytes from normal subjects and patients with congenital X-linked agammaglobulinaemia, J. Immunol., 123: 2475–2478.PubMedGoogle Scholar
- Webster, A.D.B., Rowe, M., Johnson, S., Asherson, G.L. and Harkness, A., 1979, Ecto 5’-nucleotidase deficiency in primary hypogammaglobulinaemia, in: Ciba Foundation Symposium Series No.68, p. 135–151, Elsevier, Amsterdam.Google Scholar