Evidence for a Distinct Deoxypyrimidine 5′-Nucleotidase in Human Tissues
A pyrimidine-specific 5’nucleotidase was first identified in human erythrocytes by Valentine and his colleagues during the investigation of patients with a particular form of non-spherocytic haemolytic anaemia associated with basophilic stippling of the erythrocytes (1). This nucleotidase was shown to catalyse the hydrolysis of pyrimidine 5’ribomonophosphates (2) such as uridine 5’monophosphate (UMP) and cytidine 5’monophosphate (CMP) but was found to be relatively ineffective with purine ribonucleotides such as adenosine 5’monophosphate (AMP). Subsequent examination of partially purified material from human erythrocytes (3) confirmed the identity and properties of this nucleotidase. The genetically determined enzyme deficiency is inherited as an autosomal recessive character and the enzyme is presumed to be determined by a single autosomal structural gene locus.
KeywordsHuman Erythrocyte Nucleotidase Activity Pyrimidine Nucleotide Human Gene Mapping Basophilic Stippling
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