Amino Acids, Nutrition and Stress: The Role of Biochemical Mutants in Solving Problems of Crop Quality

  • B. J. Miflin
  • S. W. J. Bright
  • S. E. Rognes
  • J. S. H. Kueh
Part of the Basic Life Sciences book series (BLSC, volume 26)


Amino acids affect crop quality in a number of ways. We consider here the deficiencies of lysine and threonine which adversely affect the nutritional quality of cereal diets and the potential role of proline in stress tolerance. To probe the biosynthesis of these amino acids further, it was decided to select for mutants of barley that synthesize enhanced amounts of them. For several reasons we considered it preferable to carry out our selection using whole embryos. The genetical and biochemical characteristics of the mutants selected are described; mutants Rothamsted (R) 906 and R4402 contain the gene aec, which causes impaired uptake of lysine; R2501 and R3202 the gene Lt1, which leads to the desensitization of one isoenzyme of aspartate kinase (AK); R3004 the gene Lt2, which causes the desensitization of another isoenzyme of AK; and R5201, R6102, and R6902 the Hypl gene, which confers resistance to hydroxyproline. R3004 and R2501 accumulate threonine and R5201, R6102, and R6902 proline.


Amino Acid Metabolism Proline Accumulation Quality Protein Maize Proline Synthesis Barley Mutant 
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Copyright information

© Plenum Press, New York 1983

Authors and Affiliations

  • B. J. Miflin
    • 1
  • S. W. J. Bright
    • 1
  • S. E. Rognes
    • 1
  • J. S. H. Kueh
    • 1
  1. 1.Biochemistry DepartmentRothamsted Experimental StationHarpenden, Herts.UK

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