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Chromosomes from Protoplasts -- Isolation, Fractionation, and Uptake

  • Russell L. Malmberg
  • Robert J. Griesbach
Part of the Basic Life Sciences book series (BLSC, volume 26)

Abstract

We have developed methods for the isolation of condensed mitotic and meiotic chromosomes from plant protoplasts. The chromosomes can be isolated from a variety of tissues: meiocytes, root tips, cell cultures, and from a variety of species: tobacco, tomato, lily, daylily, onion, and peas. The limiting steps are the ability to obtain synchronously dividing cells and conversion of those cells into protoplasts. We have also been able to demonstrate uptake of isolated chromosomes by recipient protoplasts with fluorescence microscopy at a frequency of 1%. We could partially fractionate chromosomes by size, but we also, unfortunately, were able to show that the in vitro size did not correspond well to the relative in vivo size, so the fractionation method was not noticeably useful. We have not obtained an actual genetic transformation as yet.

Keywords

Sucrose Gradient Mitotic Chromosome Meiotic Chromosome Plant Chromosome Plant Protoplast 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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    Griesbach, R.J. 1980. Ph.D. Thesis, Michigan State University, East Lansing, Michigan, U.S.A.Google Scholar
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    Griesbach, R.J., R.L. Malmberg, and P.S. Carlson. 1982. An improved technique for the isolation of higher plant chromosomes, Plant Sci. Letts. 24: 55–60.CrossRefGoogle Scholar
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    Griesbach, R.J., R.L. Malmberg, and P.S. Carlson. 1982. Uptake of isolated lily chromosomes by tobacco protoplasts, J. Hered. 73: 151–152.Google Scholar
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    Malmberg, R.L., and R.J. Griesbach. 1980. The isolation of mitotic and meiotic chromosomes from plant protoplasts. Plant Sci. Letts. 17: 141–147.CrossRefGoogle Scholar
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Copyright information

© Plenum Press, New York 1983

Authors and Affiliations

  • Russell L. Malmberg
    • 1
  • Robert J. Griesbach
    • 2
  1. 1.Cold Spring Harbor LaboratoryCold Spring HarborUSA
  2. 2.U.S. Department of AgricultureBeltsvilleUSA

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