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InVitroPlant Transformation Systems Using Liposomes and Bacterial Co-Cultivation

  • Robb T. Fraley
  • Rob B. Horsch
Part of the Basic Life Sciences book series (BLSC, volume 26)

Abstract

The development of efficient methods for introducing nucleic acids into plant cells is critical to the successful application of molecular genetic approaches to plant systems. Two methods have been developed for this purpose: 1) liposome-mediated delivery and 2) in vitro transformation of protoplasts by co-cultivation with Agrobacterium tumefaciens cells.

Liposome-mediated delivery of tobacco mosaic virus (TMV) RNA into protoplasts and resulting virus production has been used as an assay for determining incubation conditions which favor increased uptake of liposomal contents by plant cells. Under optimal conditions, the liposome method was found to be 10–1,000 times more efficient than other methods commonly used for introducing nucleic acids into plant cells.

The co-cultivation method, initially developed by Marton et al., (12) has been improved and extended to use with petunia protoplasts. In vitro transformants have been obtained with a variety of A tumefaciens strains at efficiencies near 10−1. This method has been used to obtain transformants with avirulent bacterial strains which contain disarmed Ti plasmids. The implications of both these methodologies for plant genetic engineering is discussed.

Keywords

Tobacco Mosaic Virus Virus Production Liposome Preparation Plant Protoplast Tobacco Protoplast 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1983

Authors and Affiliations

  • Robb T. Fraley
    • 1
  • Rob B. Horsch
    • 1
  1. 1.Molecular Biology DepartmentMonsanto Co.St. LouisUSA

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