Nonhistone Protein Antigens in Rat Hepatomas

  • L. S. Hnilica
  • W. N. Schmidt
  • R. C. Briggs

Abstract

Application of two dimensional Polyacrylamide gel electrophoresis to the studies on chromosomal nonhistone proteins revealed their exceptional heterogeneity (1, 2). Assuming that at least some of these proteins participate in the process of differentiation and regulation of genetic transcription, they should be cell specific. Because of their unique selectivity and sensitivity, immunological procedures are well suited for probing the cell and tissue specificity of chromosomal proteins and their structural associations. Antibodies to histones (3) and to chromosomal nonhistone proteins (4–7) were used to study chromatin structure or cellular distribution and specificity of the respective antigens. Specific antisera localized nuclear protein antigens to selected areas of polytene chromosomes (5) or to the nucleoli of normal and malignant cells (8). Isolated intact (9,10) or dehistonized (11, 12) chromatin, nucleoli (8, 13) or nuclear membrane proteins (14, 15) were all found immunogenic when injected into rabbits or other animals. Some of these antisera recognized cell or tissue-specific antigens and their changes during cell differentiation (11, 16) or carcinogenesis (17–19).

Keywords

Cellulose Filtration Albumin Urea Adenocarcinoma 

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Copyright information

© Plenum Press, New York 1983

Authors and Affiliations

  • L. S. Hnilica
    • 1
  • W. N. Schmidt
    • 1
  • R. C. Briggs
    • 1
  1. 1.Department of Biochemistry and the A.B. Hancock, Jr. Memorial LaboratoryVanderbilt University School of MedicineNashvilleUSA

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