Expression of Development-Phase Specific Alkaline Phosphatase Isoenzymes in Cultured Cancer Cells
The history of the demonstration of development-phase specific alkaline phosphatase (AP) isoenzymes goes back to 1976 when Lillian Fishman and her colleagues (1) reported their observations on placental AP. They found that the microvilli of placenta earlier than 10 weeks produced a different alkaline phosphatase than the one which characterized the second and third trimester placentas. The early placental alkaline phosphatase is heat-sensitive, inhibited by L-homoarginine, not L-phênylalanine, and migrates on electrophoresis faster than the term isoenzyme. The latter is very heat stable, inhibited by L-phenylalanine but not L-homoarginine, and is represented by a myriad of electrophoretic variants and distinctive immunologic determinants. Interestingly, the two isoenzymes overlapped in their expression between 10 and 12 weeks of pregnancy and both have the very same syncitiotropho-blast location.
KeywordsCervical Cancer Alkaline Phosphatase Sodium Butyrate Culture Cancer Cell Placental Alkaline Phosphatase
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