Variation in Expression of Human Major Histocompatibility Genes in Mouse L Cells After DNA-Mediated Gene Transfer

  • J. A. Barbosa
  • M. E. Kamarck
  • F. H. Ruddle


Somatic cell genetics and gene mapping have contributed significantly to our understanding of the organization and expression of the large and complex genomes of higher eukaryotes (1). The random segregation of donor chromosomes in somatic cell hybrids has allowed genes to be mapped to particular chromosomes by correlating their expression with the presence or absence of particular chromosomes. Recently, the availability of cloned DNA sequences and the techniques of Southern blot hybridization has circumvented the need for expression for successful gene mapping (2). From this work, a number of multigene families, i.e., groups of distinct but homologous gene sequences having similar function and structure, have been identified and chromosomally located. These gene families can either be closely linked or dispersed throughout the genome (3).


Genomic Clone Somatic Cell Hybrid Herpes Simplex Virus Thymidine Kinase Somatic Cell Genetic Major Histocompatibility Antigen 
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Copyright information

© Plenum Press, New York 1983

Authors and Affiliations

  • J. A. Barbosa
    • 1
  • M. E. Kamarck
    • 1
  • F. H. Ruddle
    • 1
  1. 1.Departments of Human Genetics and BiologyYale UniversityNew HavenUSA

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