Polypeptide Synthesis in Human Sarcoma and Normal Tissue
Over the past few years the study of quantitative changes in the proteins of transformed cell lines have mainly taken two directions. One line of experimentation has been concerned with the total cellular response to transformation (1–5), while the other has been directed to search for changes in few cellular proteins whose expression is presumed to represent primary lesions in cells transformed with DNA or RNA tumour viruses (6–9). Both experimental approaches have made use mainly of cultured cell lines. At present, the relative proportions of at least 50 polypeptides have been shown to change in transformed cell lines of human origin (1). Furthermore 13 host genes have been shown to be involved in transmission of different malignancies with different retroviruses (9). (See also other related articles in this volume).
KeywordsControl Tissue Malignant Fibrous Histiocytoma Fibrous Connective Tissue Transform Cell Line Rous Sarcoma Virus
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- 3).FORCHHAMMER, J. and KLARLUND, J. (1979). Changes in proteins from transformed cultures and tumours induced by sarcoma virus. In: Advances in Medical Oncology, Research and Education (ed. P.G. Margison) Vol. 1, p. 51, Pergamon Press, Oxford.Google Scholar
- 12).ANSORGE, W. (1981). Preparation of ultrathin gels (0.1–0.2 mm). Applications to protein separation with a new silver staining. In: International Conference in Electrophoresis, Charlston, S.C., in press.Google Scholar
- 13).MOSE LARSEN, P. (1981). An assessment of the potential offered by two dimensional gel electrophoresis and silver staining for developmental biology. (Thesis: Dept of Chemistry, Aarhus University, Denmark).Google Scholar
- 14).HAJDU, S. (1979). Pathology of soft tissue tumours. Lea and Fibiger, Philadelphia, ISBN 0–8121-0693–8.Google Scholar
- 18).FORCHHAMMER, J. (1982). Quantitative changes of some cellular polypeptides in C3H mouse following transformation by Moloney sarcoma virus. In: “Biological markers of neoplastic transformation”. (ed. P. Chandra), Plenum, in press.Google Scholar
- 21).TOPP, U.C., LANE, D. and POLLACK, R. (1980). Transformation by SV40 and polyoma virus. In: Molecular biology of tumour viruses, (ed. J. Tooze) part 2, pp. 205. Cold Spring Harbor, New York.Google Scholar
- 22).BRAVO, R., FEY, S.J., BELLATIN, J., MOSE LARSEN, P. and CELIS, J.E. (1982). Identification of a nuclear polypeptide (cyclin) whose relative proportion is sensitive to changes in the rate of cell proliferation and to transformation. In: Proceedings of the International Society of Developmental Biology, (ed. M. Burger), Alan R. Liss, New York, In press.Google Scholar