Expression of Cellular Protein in Normal and Transformed Human Cultured Cells
Malignant transformation of cultured cells is often characterized by changes in growth properties as well as of cell morphology (1–3). These alterations reflect changes in gene expression that develop through a series of progressive events (4, 5; see also article by L.M. Franks in this volume). Working with the hypothesis that malignant transformation may be due to the abnormal expression of normal genes (6–10) we have carried out a detailed and systematic study of the polypeptides synthesized by normal and transformed cells under a variety of physiological conditions in an effort to reveal cellular proteins that are involved in regulating cell proliferation and that could be used as general markers for malignant transformation (6–11). In this article we will review our studies of normal and transformed human cultured cells. These studies have so far revealed 58 transformation sensitive polypeptides that are present both in normal and transformed cells and that are common to many cell types analyzed (9). Some of these polypeptides may correspond to “oncogenes” (12–14; see also other articles in this volume), and the elucidation of their function may lead a better understanding of the processes that control cell proliferation.
KeywordsHeLa Cell Relative Proportion Embryonal Carcinoma Cell Cell Pair Human Lung Fibroblast
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