Expression of Nuclear Genes Encoding the Small Subunit of Ribulose-1,5-Bisphosphate Carboxylase
Chloroplast biogenesis requires the coordinate expression of both nuclear and chloroplast genomes. While the chloroplast genome has been shown to encode several polypeptides, structural genes for the majority of the chloroplast proteins are localized in the nucleus (see Ellis, 1981, for a review). In higher plants, the two most prominant nuclear gene products destined for the chloroplasts are the small subunit (S) of ribulose-1,5-bisphosphate carboxylase (RUBISCO) (Chua and Schmidt, 1978b; Highfield and Ellis, 1978; Cashmore et al., 1978) and thylakoid polypeptide 15, the major subunit of the light-harvesting complex (LHC) (Apel and Kloppstech, 1978; Schmidt et al., 1981). In addition to their regulation by light (Apel, 1979; Tobin and Suttie, 1980; Highfield and Ellis, 1981; Sasaki et al., 1981), nuclear genes for these polypeptides are expressed differentially in the mesophyll and bundle-sheath leaf cells of C4 plants (Broglie et al., 1982). The mechanisms for the developmental and tissue-specific regulation of these genes are unknown, although transcriptional control is likely to be a major factor.
KeywordsSmall Subunit Chloroplast Genome Transit Peptide Ribulose Bisphosphate Bisphosphate Carboxylase
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