Hydroxyproline-Rich Glycoproteins Extracted from the Cell Walls of Aerated Carrot Root Slices
Hydroxyproline containing proteins were discovered in plants in F.C. Steward’s laboratory in the 1950s (reviewed in Lamport, 1965). In 1960 Dougall and Shimbayashi and Lamport and Northcote independently reported that the bulk of the bound hydroxyproline released on acid hydrolysis was present in the cell wall (reviewed in Lamport, 1965). Since then work from several laboratories has made it clear that hydroxyproline occurs in at least three kinds of proteins -all glycoproteins. These are: 1) the cell wall proteins, 2) the arabinogalactan proteins and 3) certain lectins. These are easily separated from one another and distinguished from one another by virtue of their isoelectric points, amino acid compositions and carbohydrate compositions (reviewed in Lamport, 1981). We shall briefly review the work that has brought us to our present understanding of hydroxyproline-rich glycoproteins and present work from our laboratory on the characterization of a cell wall protein that accumulates in aerated carrot slices.
KeywordsCell Wall Protein Hydroxyproline Content Arabinogalactan Protein Anisole Butylate Hydroxy Glycine Ethyl Ester
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