Hydroxyproline-Rich Glycoproteins Extracted from the Cell Walls of Aerated Carrot Root Slices

  • J. E. Varner
  • J. B. Cooper
Part of the NATO Advanced Science Institutes Series book series (NSSA, volume 63)


Hydroxyproline containing proteins were discovered in plants in F.C. Steward’s laboratory in the 1950s (reviewed in Lamport, 1965). In 1960 Dougall and Shimbayashi and Lamport and Northcote independently reported that the bulk of the bound hydroxyproline released on acid hydrolysis was present in the cell wall (reviewed in Lamport, 1965). Since then work from several laboratories has made it clear that hydroxyproline occurs in at least three kinds of proteins -all glycoproteins. These are: 1) the cell wall proteins, 2) the arabinogalactan proteins and 3) certain lectins. These are easily separated from one another and distinguished from one another by virtue of their isoelectric points, amino acid compositions and carbohydrate compositions (reviewed in Lamport, 1981). We shall briefly review the work that has brought us to our present understanding of hydroxyproline-rich glycoproteins and present work from our laboratory on the characterization of a cell wall protein that accumulates in aerated carrot slices.


Cell Wall Protein Hydroxyproline Content Arabinogalactan Protein Anisole Butylate Hydroxy Glycine Ethyl Ester 


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  1. Akiyama, Y., M. Mori and K. Kato, 1980, 13C-NMR Analysis of Hydroxyproline Arabinosides from Nicotina tabacum. Agric. Biol. Chem. 44: 2487–2489.CrossRefGoogle Scholar
  2. Allen, A.K., N.M. Desai, A. Neuberger and J.M. Creeth, 1978, Properties of Potato Lectin and the Nature of its Glycoprotein Linkages. Biochem. J. 171: 665–674.PubMedGoogle Scholar
  3. Ashford, D., N.N. Desai, A.K. Allen, A. Ifeuberger, M.A. O’Neill and R.R. Selvendran, 1982, Structural Studies of the Carbohydrate Moieties of Lectins from Potato (Solanum tuberosum) Tubers and Thorn-apple (Datura stramonium) Seeds. Biochem. J. 201:199–208.PubMedGoogle Scholar
  4. Chao, H-Y. and W.V. Dashek, 1973, Hydroxyproline Metabolism during Mungbean and Soya-bean Seedling Growth. Ann. Bot. 37: 95–105.Google Scholar
  5. Chrispeels, M.J. and D. Sadava, 1974, Synthesis and Secretion of Proteins in Plant Cells: The Hydroxyproline-rich Glycoprotein of the Cell Wall. In Macromolecules Regulating Growth and Development, E.D. Hay, T.J. King and J. Papaconstantinon, eds. (Academic Press, New York and London) pp. 131–152.Google Scholar
  6. Chrispeels, M.T., D. Sadava and Y.P. Cho, 1974, Enhancement of Extensin Biosynthesis in Ageing Disks of Carrot Storage Tissue. J. Exp. Bot. 25: 1157–1166.CrossRefGoogle Scholar
  7. Cooper, J.B., 1982, Techniques for studying the role of hydroxyproline-rich cell wall protein. Ph.D. Thesis. Washington University, St. Louis.Google Scholar
  8. Desai, N.N., A.K. Allen and A. Neuberger, 1981, Some Properties of the Lectin from Datura stromonium (Thorn-apple) and the Nature of its Glycoprotein Linkages. Biochem. J. 197: 345–353.PubMedGoogle Scholar
  9. Dougali, D.K. and K. Shimbayashi, 1960, Factor Affecting Growth of Tobacco Callus Tissue and its Incorporation of Tyrosine. Plant Physiol. 35:396–404.CrossRefGoogle Scholar
  10. Fry, S.C., 1982, Isodityrosine, a New Crosslinking Amino Acid from Plant Cell-Wall Glycoprotein. Biochem. J. 204: 449–455.PubMedGoogle Scholar
  11. Homer, R.B. and K. Roberts, 1979, Glycoprotein Conformation in Plant cell Walls. Planta 146: 217–222.CrossRefGoogle Scholar
  12. Klis, F.M., 1976, Glycosylated Seryl Residues in Wall Protein of Elongating Pea Stems. Plant Physiol. 57:274–276.CrossRefGoogle Scholar
  13. Lamport, D.T.A. and D.H. Northcote, 1960, Hydroxyproline in Primary Cell Walls of Higher Plants. Nature 188: 665–666.CrossRefGoogle Scholar
  14. Lamport, D.T.A., 1965, The Protein Component of Primary Cell Walls. In Advances in Botanical Research, R.D. Preston, ed. (Academic Press, London and New York), pp. 151–218.Google Scholar
  15. Lamport, D.T.A. and D.H. Miller, 1971, Hydroxyproline Arabinosides in the Plant Kingdom. Plant Physiol. 48: 454–456.PubMedCrossRefGoogle Scholar
  16. Lamport, D.T.A., 1977, Structure, Biosynthesis and Significance of Cell Wall Glycoproteins. In Recent Advances in Phytochemistry, Vol. II, F.A. Loewus and V.C. Runeekles, eds. (Plenum Press, New York) pp. 79–115.Google Scholar
  17. Lamport, D.T.A. and J.W. Catt, 1981, Glycoproteins and Enzymes of the Cell Wall. In Plant Carbohydrates II, Encyclopedia of Plant Physiology, New Series 13B, W.A. Tanner and F.A. Loewus, eds. (Springer-Verlag, Berlin, Heidelberg, New York) pp. 133–165.Google Scholar
  18. Lang, W., 1976, Biosynthesis of Extensin During Normal and Light-Inhibited Elongation in Radish Hypocotyls. Z. Pflanzenphysiol. 78: 228–235.Google Scholar
  19. Leach, J.E., 1981, Localization, characterization, and quantification of a bacterial agglutinin from potatoes. Ph.D. Thesis. University of Wisconsin, Madison.Google Scholar
  20. Miller, D.H., D.T.A. Lamport, and M. Miller, 1972, Hydroxyproline Heterooligosaccharides in Chlamydomonas. Science 176: 918–920.PubMedCrossRefGoogle Scholar
  21. Pike, C.S., H. Un, J.C. Lystash, and A.M. Showalter, 1979, Phytochrome Control of Cell Wall-bound Hydroxyproline Content in Etiolated Pea Epicotyls. Plant Physiol. 63: 444–449.PubMedCrossRefGoogle Scholar
  22. Ridge, I., and D.J. Osborne, 1970, Hydroxyproline and Peroxidases in Cell Walls of Pisum sativum: Regulation by Ethylene. J. Exp. Bot. 21: 843–856.CrossRefGoogle Scholar
  23. Roberts, K., 1974, Crystalline Glycoprotein Cell Walls of Algae: Their Structure, Composition and Assembly. Phil. Trans. R. Soc. Lond. B 268: 129–146.CrossRefGoogle Scholar
  24. Roberts, K., 1979, Hydroxyproline—It’s Assymetric Distribution in a Cell Wall glycoprotein. Planta 146: 275–280.CrossRefGoogle Scholar
  25. Sadava, D., and M.J. Chrispeels, 1978, Synthesis and Secretion of Cell Wall Glycoprotein in Carrot Root Discs. In Biochemistry in Wounded Plant Tissues. G. Kahl, ed. (Walter de Grupter, Berlin, New York) pp. 85–102.Google Scholar
  26. Steward, F.C, R.H. Wetmore, J.F. Thompson and J.P. Nitsch, 1954, A Quantitative Chromatographie Study of Nitrogenous Components of Shoot Apices. Am. J. Bot. 41: 123–134.CrossRefGoogle Scholar
  27. Steward, F.C., J.F. Thompson, and J.K. Pollard, 1958, Contrasts in the nitrogenous Composition of Rapidly Growing and Non-growing Plant Tissues. J. Exp. Bot. 25: 1–10.CrossRefGoogle Scholar
  28. Stuart, D.A. and J.E. Varner, 1980, Purification and Characterization of a Salt-extractable Hydroxyproline-Rich Glycoprotein from Aerated Carrot Discs. Plant Physiol. 66: 787–792.PubMedCrossRefGoogle Scholar
  29. Stuart, D.A., T.J. Mozer and J.E. Varner, 1982, Cytosine-Rich Messenger RNA from Carrot Root Discs. Biochem. Biophys. Res. Comm. 105: 582–588.PubMedCrossRefGoogle Scholar
  30. Tanaka, M., K. Sato, and T. Uchida, 1981, Plant Prolyl Hydroxylase Recognizes Poly(L-proline)II Helix. J. Biol. Chem. 256: 11397–11400.PubMedGoogle Scholar
  31. Toppan, A., D. Roby and M-T Esquerre-Tugaye, 1982, Cell Surfaces in Plant-Microorganism Interactions. III. In Vivo Effect of Ethylene on Hydroxyproline-Rich Glycoprotein Accumulation in the Cell Wall of Diseased Plants. Plant Physiol. 70: 82–86.Google Scholar
  32. VanHolst, G.J., F.M. Klis, F. Bouman and D. Stegwee, 1980, Changing Cell-Wall Compositions in Hypocotyls of Dark-grown Bean Seedlings. Planta 149: 209–212.CrossRefGoogle Scholar
  33. VanHolst, G.J., F.M. Klis, P.J.M. deWildt, C.A.M. Hazenberg, J. Buijs and D. Stegwee, 1981, Arabinogalactan Protein from a Crude Cell Organelle Fraction of Phaseolus vulgaris L. Plant Physiol. 68: 910–913.CrossRefGoogle Scholar
  34. VanHolst, G.J. and F.M. Klis, 1981, Hydroxyproline Glycosides in Secretory Arabinogalactan-Protein of Phaseolus vulgaris L. Plant Physiol. 68: 979–980.CrossRefGoogle Scholar
  35. Vaughan, D., and E. Cusens, 1974, Protein-Bound Hydroxyproline and Root Extension Growth. Biochem. Soc. Trans. 2: 124–126.Google Scholar

Copyright information

© Plenum Press, New York 1983

Authors and Affiliations

  • J. E. Varner
    • 1
  • J. B. Cooper
    • 1
  1. 1.Department of BiologyWashington UniversitySt. LouisUSA

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