The Potential of Cauliflower Mosaic Virus as a Gene Vector
Cauliflower mosaic virus (CaMV) is the type member of the caulimoviruses, a group of plant viruses which have genomes of double-stranded DNA. CaMV is the most studied member of the group as it can be purified most easily. The encapsidated viral DNA is a circular molecule of 8Kbp which has two unusual features. Many of the molecules have a twisted conformation (Hull et al., 1976; Hull, 1981; Hohn et al., 1982) which is not due to them being covalently closed. The DNA of all CaMV isolates has site specific discontinuities (Hull & Howell 1978; Volovitch et al., 1978) which have been shown by sequencing to be overlaps (Franck et al., 1980; Richards et al., 1981). Most CaMV isolates have three discontinuities (gaps), one [G1] in one strand (the α strand) (Fig 1) and two [G2 and G3] in the other, giving the β and Ϫ strands. One isolate (CM4-184) which has a deletion in the region of one discontinuity [G3] thus has only two discontinuities, one in each strand (Hull & Howell, 1978). The DNAs of other caulimoviruses also have discontinuities, all with one in one strand, some with two in the complementary strand, others with three (Hull and Donson, 1982).
KeywordsGene Vector Cauliflower Mosaic Virus Circular Molecule Inclusion Body Protein Figwort Mosaic Virus
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- Hull, R. 1981. Cauliflower mosaic virus DNA as a possible gene vector for higher plants. In Genetic Engineering in the Plant Sciences, ed. N.J. Panopoulos, pp 99-109, Praeger Scientific.Google Scholar
- Shepherd, R.J., Gronenborn, B., Gardner, R. & Daubert, S.D. 1981. Molecular cloning of foreign DNA in plants using cauliflower mosaic virus as a recombitant vector. In Genetic Engineering in the Plant Sciences, ed. N.J. Panapoulos, pp. 255-257. Praeger Scientific.Google Scholar