DNA Polymerase and DNA Synthesis in Wheat Embryo Mitochondria
The study of mitochondria isolated from differenct sources has shown that these organelles possess a unique DNA. In the case of mitochondria from lower eucaryotes or animal cells this DNA is a double stranded circular molecule1. In animal cells, DNA polymerase γ, the enzyme involved in the replication of the organelle genome, has been studied in some detail2–5. The mitochondria of higher plants perform the same function as the organelles from other sources. However, the size of the mitochondrial DNA from plants is much bigger than its animal counterpart6. We have previously reported a preliminary study of the wheat mitochondrial DNA polymerase7. This enzyme differs in some of its properties from the animal mitochondrial DNA polymerase γ. In this communication we present further studies of the highly purified wheat mitochondrial DNA polymerase, as well as the results we have obtained with a DNA synthesizing system using whole purified wheat mitochondria.
KeywordsWheat Embryo Organelle Genome Animal Counterpart6 Wheat Mitochondrion Template Poly
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