A Search for Nodulin Genes of Soybean
The development of root nodule organs and eventual symbiotic nitrogen fixation in a legume-Rhizobium association is the result of complex genetic interactions between the two organisms. Data accumulated over 4 decades has shown that the host plays an important role in this process. The influence of the host plant is observed at all levels: recognition and acceptance of the rhizobial strain; temporal regulation of nodule development; the number, size and gross morphology of nodules; the intracellular organization and structure of nodule cells; and finally the activity of the functional nodule (see Nutman, 1981; Verma, 1981; Verma and Long, 1982). These processes are regulated in part by a number of host genes, and several plant mutants have been isolated from various species which exhibit specific perturbations of nodule development and efficiency in fixing atmospheric nitrogen (Caldwell & West, 1977; Nutman, 1981). However, the exact number and function of these genes is not known. Using molecular and immunological techniques, we have attempted to identify some of the host genes and their products which may be involved in the development of this symbiotic state. The antibodies raised against soluble proteins of soybean root nodules, when adsorbed with uninfected (control) root proteins and reacted to the 35S-methionine labelled in vitro translation products of host polysomes, yielded a group of polypeptides which have been termed nodulins (Legocki and Verma, 1980). Some of these proteins, including nodulin-35 (Legocki and Verma, 1979), appear to be induced in parallel with leghemoglobins (Lbs) and have been implicated in the process of symbiosis (Verma, 1980).
KeywordsHost Gene EcoRI Fragment Nodule Development Strain 6lA76 NODULIN Gene
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