The In Vivo Transcriptional Activity of the Barley Chloroplast Genome

  • Carsten Poulsen
Part of the NATO Advanced Science Institutes Series book series (NSSA, volume 63)


Well characterized genes localized on chloroplast DNA* include those for the large subunit of ribulose bisphosphate carboxylase1,2,3 and the photogene 321,4,5. Also located on chloroplast DNA are the genes for the α-, β- and ε-subunits of the CF1 ATP synthetase and the photosystem II associated chlorophylla-proteins 2 and 36. Work on the localization of the genes for chlorophylla-protein 1 of photosystem I7 and cytochrome f8 is in progress. The genes for chloroplast ribosomal 16S, 23S, 5S and 4.5S RNSs have been extensively mapped1,9,10 as have genes for many chloroplast tRNA species1,11,12.


Chloroplast Genome Ribulose Bisphosphate HindIII Fragment Dark Grown Seedling Small Single Copy 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



large subunit




kilobase (s)


chloroplast DNA


chloroplast RNA


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  1. 1.
    L. Bogorad, S.O. Jolly, G. Link, L. McIntosh, C. Poulsen, Z. Schwarz and A. Steinmetz, Studies of the maize chloroplast chromosome, in: “Biological chemistry of organelle formation”, T. Bücher, W. Sebald and H. Weiss, eds., Springer-Verlag, Berlin, Heidelberg, New York (1980).Google Scholar
  2. 2.
    L. McIntosh, C. Poulsen and L. Bogorad, Chloroplast gene sequence for the large subunit of ribulose bisphosphate carboxylase of maize, Nature 288:556–560 (1980).CrossRefGoogle Scholar
  3. 3.
    B. Koller, H. Delius and T.A. Dyer, The organization of the chloroplast DNA in wheat and maize in the region containing the LS gene, Eur. J. Biochem. 122: 17–23 (1982).PubMedCrossRefGoogle Scholar
  4. 4.
    K.E. Steinback, L. McIntosh, L. Bogorad and C.J. Arntzen, Identification of the triazine receptor protein as a chloroplast gene product, Proc. Natl. Acad. Sei. USA, 78:7463–7467 (1981).CrossRefGoogle Scholar
  5. 5.
    G. Link, Phytochrome control of plastid mRNA in mustard (Sinapis alba L.) (1982).Google Scholar
  6. 6.
    D. von Wettstein, Chloroplast and nucleus: conterted interplay between genomes of different cell organelles. The Emil Heitz Lecture, in: “International Cell Biology 1980–1981”, H.G. Schweiger, ed., SpringerVerlag, Berlin, Heidelberg (1981).Google Scholar
  7. 7.
    R. Nechushtai, N. Nelson, A.K. Mattoo and M. Edelman, Site of synthesis of subunits to photosystem 1 reaction center and proton-ATPase in Spirodela, FEBS lett. 125:115–119 (1981).CrossRefGoogle Scholar
  8. 8.
    A. Doherty and J.C. Gray, Synthesis of cytochrome f by isolated pea plastids, Eur. J. Biochem. 98:87–92 (1979).PubMedCrossRefGoogle Scholar
  9. 9.
    C.M. Bowman, B. Koller, H. Delius and T.A. Dyer, A physical map of wheat chloroplast DNA showing the locations of the structural genes for the ribosomal RNAs and the large subunit of ribulose 1,5-bisphosphate carboxylase, Mol. Gen. Genet. 183:93–101 (1981).CrossRefGoogle Scholar
  10. 10.
    F. Takaiwa and M. Sugiara, The complete sequence of a 23-S rRNA from tobacco chloroplasts, Eur. J. Bio, chem. 124:13–19 (1982).Google Scholar
  11. 11.
    A.J. Driesel, E.J. Crouse, K. Gordon, H.J. Bohnert, R.G. Herrmann, A. Steinmetz, M. Mubumbila, M. Keller, G. Burkard and J.H. Weil, Fractionation and identification of spinach chloroplast tRNAs and mapping of their genes on the restriction map of chloroplast DNA, Gene 6:285–306 (1979).PubMedCrossRefGoogle Scholar
  12. 12.
    Z. Schwarz, S.O. Jolly, A.A. Steinmetz and L. Bogorad, Overlapping divergent genes in the maize chromosome and in vitro transcription of the gene for tRNAHis, Proc. Natl. Acad. Sei. USA 78:3423–3427 (1981).CrossRefGoogle Scholar
  13. 13.
    C. Poulsen, Carlsberg Res. Commun, in preparation.Google Scholar
  14. 14.
    X. Soberon, L. Covarrubias and F. Bolivar, Construction of new cloning vehicles. IV. Deletion derivatives of pBR322 and pBR325, Gene 9:287–305 (1980).PubMedCrossRefGoogle Scholar
  15. 15.
    J.R. Bedbrook and L. Bogorad, Endonuclease recognition sites mapped on Zea mays chloroplast DNA, Proc. Natl. Acad. Sei. USA 73:4309–4313 (1976).CrossRefGoogle Scholar
  16. 16.
    E.M. Southern, Detection of specific sequences among DNA fragments separated by gel electrophoresis, J. Mol. Biol. 98:503–517 (1975).PubMedCrossRefGoogle Scholar
  17. 17.
    J.C. Alwine, D.J. Kemp, B.A. Parker, J. Reiser, J. Renart, G.R. Stark and G.M. Wahl, Detection of specific RNAs or specific fragments of DNA by fractionation in gels and transfer to diazobenzyloxymethyl paper, in: “Methods in Enzymology”, vol. 68, R. Wu, ed. (1980).Google Scholar
  18. 18.
    P.W. Rigby, M. Dieckmann, C. Rhodes and P. Berg, Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I, J. Mol. Biol. 113:237–251 (1977).PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1983

Authors and Affiliations

  • Carsten Poulsen
    • 1
  1. 1.Department of PhysiologyCarlsberg LaboratoryCopenhagen ValbyDenmark

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