Abstract
Chloroplasts contain elongation factors for protein synthesis different from the corresponding factors involved in cytoplasmic and mitochondrial protein synthesis1. Previously, we have demonstrated that chloroplast elongation factor G is synthesized in the organelle in Chlorella vulgaris 2 and that this factor, as well as elongation factor Tu (EF-Tuchl), are synthesized in spinach chloroplasts3. In addition, preliminary results were presented suggesting that the two elongation factors are coded in spinach chloroplast DNA4. We present now a more rigorous evidence that EF-Tuchl is coded in the organellar DNA.
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References
O. Ciferri, O. Tiboni, M.L. Munoz-Calvo and G. Camerino, Protein synthesis in plants: specif icy and role of the cytoplasmic and organellar systems, in “Nucleic acids and protein synthesis in plants”, L. Bogorad and J.H. Weil, eds., p. 155 Plenum, New York, 1977.
O. Ciferri, and O. Tiboni Evidence for the synthesis in the chloroplast of elongation factor G, Plant Sei. Lett. 7: 455 (1976).
O. Ciferri, G. Di Pasquale and O. Tiboni Chloroplast elongation factors are synthesized in the chloroplast, Eur. J. Biochem. 102: 331 (1979).
O. Ciferri, O. Tiboni, G. Di Pasquale and D. Carbonera, Site of synthesis and codification of chloroplast elongation factors, in “Genome organization and expression in plants”, C.J. Leaver, ed., Plenum, New York, p. 373 (1980).
O. Tiboni, G. Di Pasquale, and O. Ciferri Purification of the elongation factors present in spinach chloroplasts, Eur. J. Biochem. 92: 471 (1978).
D. W. Cleveland, S. G. Fisher, and M. W. Kirschner, and U. K. Laemmli Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis J. Biol. Chem. 252: 1102 (1977).
L. L. Spremulli Chloroplast elongation factor Tu: evidence that it is the product of a chloroplast gene in Euglena, Arch. Biochem. Biophys. 214: 734 (1982).
J. C. Watson, and S. J. Surzycki Extensive sequence homology in the DNA coding for elongation factor Tu from Escherichia coli and the Chlamydomonas reinhardtii chloroplast, Proc. Natl. Acad. Sei. U.S.A. 79: 2264 (1982).
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© 1983 Plenum Press, New York
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Ciferri, O., Tiboni, O., Di Pasquale, G. (1983). Chloroplast EF-Tu is Coded in Spinach Chloroplast DNA. In: Ciferri, O., Dure, L. (eds) Structure and Function of Plant Genomes. NATO Advanced Science Institutes Series, vol 63. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4538-1_19
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DOI: https://doi.org/10.1007/978-1-4684-4538-1_19
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