Organization and Structure of the Genes for the β and ε Subunits of Spinach and Pea Chloroplast ATPase
Chloroplast ATPase (CF1) is composed of five subunits, three of which (α,β and ε) are synthesized within the chloroplast (see (1) for references) and coded by chloroplast genes (2). The γ and δ subunits are synthesized in the cytoplasm and are presumed to be coded by nuclear genes (see (1) for references). The genes for the β and ε subunits map close to the gene (rbcL) for the large subunit of ribulose bisphosphate carboxylase but the gene for the α subunit is located some 40 kb away on the spinach chloroplast DNA (cpDNA) map (2). In this paper we report the results of our investigations into the structure and organization of the genes for the β and ε subunits of ATPase from Spinacia oleracea and Pisum sativum chloroplasts.
KeywordsatpB Gene Maize Chloroplast Chloroplast ATPase Dicistronic mRNA Spinach Plastid Chromosome
Unable to display preview. Download preview PDF.
- 5.Zurawski, G., Bottomley, W. and Whitfeld, P.R., Structure of the genes for the β and ε subunits of spinach chloroplast ATPase indicates a dicistronic mRNA and an overlapping translation stop/start signal. Proc. Natl. Acad. Sei. USA In Press.Google Scholar
- 7.Saraste, M., Gay, N.J., Eberle, A., Runswick, M.J. and Walker, J.E., The atp operon: nucleotide sequence of the genes for the γ,β and ε subunits of Escheriehia coli ATP synthase. Nucleic Acids Res. 9: 5287 (1981).Google Scholar