Head-Space/Gas-Liquid Chromatography in Clinical Microbiology with Special Reference to the Laboratory Diagnosis of Urinary Tract Infections
Head-space/gas-liquid chromatography (HS/GLC) has been used in food microbiology since 1965. So far it has not been widely applied in clinical microbiology; in fact, considerably less than has GLC using other methods of sample preparation. This is surprising because HS samples can be easily prepared and quickly analyzed, and HS/GLC yields chromatograms that are readily interpreted, three valuable qualities in a busy clinical laboratory.
KeywordsUrinary Tract Infection Volatile Compound Metabolic Product Clinical Microbiology Urine Specimen
Unable to display preview. Download preview PDF.
- Beezer, A. E., Bettelheim, K. A., Newell, R. D., and Stevens J., 1974, Diagnosis of bacteriuria by flow microcalorimetry, Science Tools 21:13.Google Scholar
- Johnston, H. H., Mitchell, C. J., and Curtis, G. D. W., 1976, An automated test for the detection of significant bacteriuria, Lancet ii:400.Google Scholar
- Larsson, L., Mårdh, P.-A., and Odham, G., 1978b, Analysis of amines and other bacterial products by head-space gas chromatography, Acta Pathol. Microbiol. Scand., Sect. B. 86:207.Google Scholar
- Larsson, L., Hoist, E., Gemmell, C. G., and Mårdh, P.-A., 1980a, Characterization of Clostridium difficile and its differentiation from Clostridium sporogenes by automatic head-space gas chromatography, Scand. J. Infect. Dis. Suppl. 22:37.Google Scholar
- Larsson, L., Mårdh, P.-A., and Odham, G., 1981, Automated head-space gas chromatography in the identification of anaerobes; and of bacteria encountered in urinary tract infections, 3rd International Symposium on Rapid Methods and Automation in Microbiology, Abstracts No. 54.Google Scholar
- Murray, P. R., and Niles, A. C., 1981, Detection of bacteriuria: Manual screening test and early examination of agar plates, I. Clin. Microbiol. 13:85.Google Scholar