Construction and Transfer into Mammalian Cells of a Vector Containing Insect Histone Genes
As illustrated by other articles in this volume, with the advent of recombinant DNA technology and methods for introducing functional foreign genes into many types of eukaryotic cells, a revolution has occurred in the level of our understanding of the way genes are regulated in living cells. Nonetheless, in spite of the impressive progress made concerning the role of DNA sequence in functions such as promotion, enhancement, initiation of transcription, splicing, polyadenylation and termination, much remains to be learned. It is worth considering, for example, whether such information, by itself, will enable us to understand the subtleties of gene expression and regulation known to occur during complex cellular processes such as development and differentiation. For, in addition to the obvious importance of DNA sequence in the regulation of gene activity in eukaryotic cells, it is very likely that genomic function is also regulated by the structure and composition of the chromatin itself1–4. Thus, it seems reasonable to predict that a complete knowledge of the mechanisms regulating genomic activity in eukaryotic cells will only come when we understand both the structure and function of all the various components of chromatin.
KeywordsProtoplast Fusion Histone Gene Tissue Culture Cell Chloramphenicol Acetyl Transferase Chloramphenicol Acetyl Transferase Activity
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