Close Linkage of Transferred Galactokinase and Thymidine Kinase Genes in a Transformant after DNA-Mediated Gene Transfer
There are many similarities and some differences between DNA- and chromosome-mediated gene transfer. The cellular uptake of both DNA1 and metaphase chromosomes2 involves phagocytosis and there is a requirement for co-precipitation of the donor DNA3 or chromosomes4 with calcium phosphate to achieve optimal frequencies of gene transfer. In either method, transformants usually initially exhibit an unstable phenotype, and stabilization ultimately occurs through covalent integration of the donor DNA with recipient chromosomal DNA at multiple non-homologous sites. The optimal frequency for gene transfer is dependent upon many factors but a higher frequency of transfer has been reported for chromosome-than DNA-mediated gene transfer under similar conditions.5
KeywordsThymidine Kinase Primary Transformant Chinese Hamster Cell Serial Transfer Thymidine Kinase Gene
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