DNA Transfer into Animal Cells

  • Axel C. Garapin
  • Florence Colbère-Garapin
Part of the NATO Advanced Science Institutes Series book series (NSSA, volume 61)


The introduction or the reintroduction of cloned genes into eukaryotic recipients is somewhat of a paradox. It is often necessary to check the functional integrity of a cloned cDNA or chromosomal gene, or to study the expression of a gene normally in the repressed state. The complementation of defective animal cells can be studied in vitro by gene transfer.


Thymidine Kinase Mouse Mammary Tumor Virus Dihydrofolate Reductase Thymidine Kinase Gene Male Pronucleus 
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  1. Alt, F., Beatino, R. and Schink R.T., 1978, Selective multiplication of dihydrofolate reductase genes in methrothrexate resistant variants of cultured murine cells — J. Biol. Chem., 253 : 1357.PubMedGoogle Scholar
  2. Brinster, R.L., Chen, H.Y., Trumbauer, M., Senear, A.W., Warren, R. and Palmiter, R.D., 1981, Somatic expression of herpes thymidine kinase in mice following infection of a fusion gene into eggs — Cell, 27 : 223PubMedCrossRefGoogle Scholar
  3. Capecchi, M.R., 1980, High efficiencyl transformation by direct microinjection of DNA into cultured mammalian cells — Cell, 22 : 479.PubMedCrossRefGoogle Scholar
  4. Colbère-Garapin, F., Hordonicean, F., Kourilsky, P. and Garapin, A.C., 1981, A new dominant hybrid selective marker for higher eukaryotic cells — J. Mol. Biol., 150 : 1.PubMedCrossRefGoogle Scholar
  5. Colbère-Garapin, F., Chousterman, S., Horodniceanu, F., Kourilsky, P. and Garapin, A.C., 1979, Cloning of the active thymidine kinase gene of herpes virus simplex type I in E. coli K12 — Proc. Natl. Acad. Sci., U.S.A., 76 : 3755.PubMedCrossRefGoogle Scholar
  6. Costantini, F. and Lacy, E., 1981, Introduction of a rabbit β-globin gene into the mouse germ line — Nature (London) 294 : 92.CrossRefGoogle Scholar
  7. Di Maio, D., Treisman, R. and Maniatis, L., 1982, Bovine papillomavirus vector that propagates as a plasmid in both mouse and bacterial cells — Proc. Natl. Acad. Sci., U.S.A., 79 : 4030.CrossRefGoogle Scholar
  8. Fraley, R., Subramani, S., Berg, P., and Papahadjopoulos, D., 1980, Introduction of liposome encapsulated SV 40 DNA into cells — J. Biol. Chem., 255 : 10431PubMedGoogle Scholar
  9. Gluzman-Yakov, 1981, SV 40 transformed simian cells support the replication of early SV 40 mutants — Cell : 23 : 175.CrossRefGoogle Scholar
  10. Graessmann, M. and Graessmann, A., 1976, Early simian virus 40 specific RNA contains information of tumor antigen formation and chromatin replication — Proc. Natl. Acad. Sci., U.S.A., 77 : 7380.Google Scholar
  11. Graham, F.L. and Van der Eb, A., 1973, A new technique for the assay of infectivity of human adenovirus 5 DNA — Virology, 52 : 456.PubMedCrossRefGoogle Scholar
  12. Gruss, P. and Khoury, G., 1982, Gene transfer into mammalian cells : use of viral vectors to investigate regulatory signals for the expression of eukaryotic genes — Current Topics in Microbiol. and Immunol., 96, Henk, W., Ed. — Springer Verlag, Berlin.Google Scholar
  13. Kit, P. and Dubbs, D.R., 1963, Acquisition of thymidine kinase activity by herpes simplex infected mouse fibroblast cells — Biochem. Biophys. Res. Commun., 11 : 55.CrossRefGoogle Scholar
  14. Lee, F., Mulligan, R., Berg, P. and Ringold, G., 1981, Glucocorticoids regulate expression of dihydrofolate reductase cDNA in mouse mammary tumor virus chimaeric plasmids — Nature (London) 294 : 228.CrossRefGoogle Scholar
  15. Millman, G. and Herzberg, M., 1981, Efficient DNA transfection and rapid assy for thymidine kinase activity and viral antigenic determinants — Somat. Cell. Genet., 7 : 161.CrossRefGoogle Scholar
  16. Mulligan, R.C., Howard, B.H. and Berg, P., 1979, Synthesis of rabbit β-globin in cultured monkey kidney cells following infection with a SV 40 β-globin recombinant genome — Nature (London) 277 : 108.CrossRefGoogle Scholar
  17. Munyon, W., Kraiselburd, E., Davis, D. and Mann, J., 1971, Transfer of thymidine kinase to thymidine kinaseless L cells by infection with ultraviolet irradiated herpes simplex virus — J. Virol., 7 : 1971.Google Scholar
  18. Mocarski, E., Post, L.E. and Roizman, B., 1980, Molecular engineering of the herpes simplex virus genome insertion of a second L-S function into the genome causes additional genome inversions. Cell, 22 : 243.PubMedCrossRefGoogle Scholar
  19. Papahadjopoulos, D., Mayhow, E., Poste, G., Smith, and Vail, W.J., 1974, Incorporation of lipid vesicles by mammalian cell provides a potential method for modifying cell behavior — Nature (London) 252 : 163.Google Scholar
  20. Perucho, M., Hanahan, D. and Wigler, M., 1980, Genetic and physical linkage of exogeneous sequences in transformed cells — Cell, 22 : 309.PubMedCrossRefGoogle Scholar
  21. O’Hare, K., Benoist, C. and Breathnach, R., 1981, Transformation of mouse fibroblasts to methrotrexate resistance by a recombinant plasmid expressing a prokaryotic dihydrofolate reducatase — Proc. Natl. Acad. Sci., U.S.A., 78 : 1527.PubMedCrossRefGoogle Scholar
  22. Rassoulzadegan, M., Binetruy, B. and Cuzin, F., 1982, High frequency of gene transfer after fusion between bacteria and eukaryotic cells — Nature (London) 295 : 257.CrossRefGoogle Scholar
  23. Rusconi, S. and Schaffner, 1981, Transformation of frog embryos with a rabbit β-globin gene — Proc. Natl. Acad. Sci., U.S.A., 78 : 5051.PubMedCrossRefGoogle Scholar
  24. Sandri-Goldin, R., Goldin, A., Levine, M. and Glorioso, J.C., 1981, High frequency transfer of cloned herpes simplex virus type 1 sequences to mammalian cells by protoplast fusion — Mol. Cell. Biol., 1 : 743.PubMedGoogle Scholar
  25. Schaffner, W., 1980, Direct transfer of cloned genes from bacteria to mammalian cells — Proc. Natl. Acad. Sci., U.S.A., 77 : 2163.PubMedCrossRefGoogle Scholar
  26. Sarver, N., Gruss, P., Law, M.F., Khoury, G. and Howley, P.M., 1981, Bovine papillomavirus deoxyribonucleic acid : a novel eukaryotic cloning vector — Mol. Cell. Biol., 1 : 486.PubMedGoogle Scholar
  27. Wagner, E.F., Stewart, T.A. and Mintz, B., 1981, The human β-globin gene and a functional viral thymidine kinase gene in developing mice — Proc. Natl. Acad. Sci., U.S.A., 78 : 5016.PubMedCrossRefGoogle Scholar
  28. Wahl, G.M., Padjett, R.A. and Stark, G.R., 1979, Gene amplification causes overproduction of three enzymes of UMP in N-(phosphona-cetyl)-L-aspartate resistant hamster cell — J. Biol. Chem., 254 : 8679.PubMedGoogle Scholar
  29. Wigler, M., Silverstein, S., Lee, L.S., Pellicer, A., Cheng, Y.C. and Axel, R., 1977, Transfer of purified herpes virus thymidine kinase gene to cultured mouse cells — Cell, 11 : 223.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1983

Authors and Affiliations

  • Axel C. Garapin
    • 1
  • Florence Colbère-Garapin
    • 1
  1. 1.Biologie Moléculaire du Gène et Virologie MédicaleInstitut PasteurParis Cédex 15France

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