Transformation and Storage of Competent Yeast Cells
Since the transformation of yeast was first demonstrated using hybrid Plasmids1, several procedures have been developed which allow yeast to be transformed with plasmid DNA1,2,3. A new transformation procedure has been recently developed (K. Murata, in press) which reprsents a radical departure from previously published methods. This procedure employs lithium acetate instead of exogenous enzymes (i.e. glusulase, lyticase, zymolyase) to make yeast cells competent for transformation. As a result of this and other changes, yeast can be transformed more rapidly and more reproducibly. Furthermore, the time required for the detection of transformants is dramatically reduced. We have adapted this procedure to allow the storage of competent yeast cells for up to 2 weeks at -70°C without significant loss of transformation efficiency.
KeywordsYeast Cell Transformation Efficiency Competent Cell Transformation Procedure Lithium Acetate
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