Use of Transformation and Meiotic Gene Conversion to Construct a Yeast Strain Containing a Deletion in the Alcohol Dehydrogenase I Gene
Techniques have been developed using in vitro mutagenesis and yeast transformation to alter the genetic material of the yeast Saccharomyces cerevisiae (1). Features of the yeast system which make this possible include the following: the availability of selectable markers; the ease of transformation of yeast with exogenous DNA; the integration of DNA into yeast chromosomes by homologous recombination; and the ease of genetic manipulation of yeast cells. As an illustration of some of the features of the yeast system, we describe the construction of a yeast strain which contains a deletion of the 5′ end of the alcohol dehydrogenase I (ADHI) gene (ADC1) of yeast. Deletions are, in general, rare events in yeast so the ability to introduce in vitro created deletions into the yeast genome is quite useful.
KeywordsYeast Strain Alcohol Dehydrogenase Gene Conversion Yeast Genome Allyl Alcohol
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