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Approaches to Plant Protoplast Transformation Using Drug Resistance and Auxotroph Complementation as Selective Markers

  • R. D. Shillito
  • G. Lazar
  • J. Paszkowski
  • K. Shimamoto
  • Z. Nicola-Koukolikova
  • B. Hohn
  • T. Hohn
  • I. Potrykus
Part of the NATO Advanced Science Institutes Series book series (NSSA, volume 61)

Summary

The factors involved in building a successful plant protoplast transformation system are discussed, with particular reference to the methods of delivery, choice of markers and expression of introduced DNA. Experiments aimed at transforming protoplasts with resistance and other genes are described.

Keywords

Selectable Marker Crown Gall Eukaryotic Expression Vector Cointegrate Vector Tobacco Protoplast 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1983

Authors and Affiliations

  • R. D. Shillito
    • 1
  • G. Lazar
    • 1
  • J. Paszkowski
    • 1
  • K. Shimamoto
    • 1
  • Z. Nicola-Koukolikova
    • 1
  • B. Hohn
    • 1
  • T. Hohn
    • 1
  • I. Potrykus
    • 1
  1. 1.Friedrich Miescher-InstitutBaselSwitzerland

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