Plant Genetic Manipulations: Applications from Plant Somatic Cell Genetics
Currently there is much interest in the extent to which studies in plant somatic cell genetics will contribute to plant genetic manipulations and thereby to crop improvement in general. Aspects of plant genetic manipulations have recently been reviewed, and the special role of protoplasts in this respect have been highlighted2. In this survey some of these aspects will be further discussed in the light of more recent work mainly from our Genetic Manipulation Group here at Nottingham, but other work will be discussed where relevant.
KeywordsNitrate Reductase Somatic Hybridization Protoplast Fusion Mesophyll Protoplast Plant Protoplast
Unable to display preview. Download preview PDF.
- 2.E. C. Cocking, Somatic hybridization by the fusion of isolated protoplasts — an alternative to sex, in; “Plant Cell and Tissue Culture Principles and Applications”, W. R. Sharp et al., ed., Ohio State University Press, Columbus (1979).Google Scholar
- 5.E. C. Cocking, Selection and somatic hybridisation, in: “Frontiers of Plant Tissue Culture”, T. A. Thorpe, ed., Int. Assoc. Plant Tissue Culture (1978).Google Scholar
- 6.J. B. Power, S. F. Berry, J. V. Chapman, and E. C. Cocking, Somatic hybridisation of sexually incompatible Petunias: Petunia parodii, Petunia parviflora, Theor. Appl. Genet. 57:1 (1980).Google Scholar
- 7.E. C. Cocking, Plant cell hybrids and somatic hybrid plants, in: “Chromosomes Today, Vol.7”, George Allen and Unwin (1981).Google Scholar
- 8.Z-H. Xu, M. R. Davey, and E. C. Cocking, Isolation and sustained division of Phaseolus aureus (Mung Bean) root protoplasts, Z. für Pflanzenphvsiol. 104:289 (1981).Google Scholar
- 10.D. Y. Lu, D. Pental, and E. C. Cocking, Plant regeneration from seedling cotyledon protoplasts, Z. für Pflanzenphysiol. 107:59 (1982).Google Scholar
- 11.A. Morgan and E. C. Cocking, Plant regeneration from protoplasts of Lycopersicon esculentum Mill., Z. für Pflanzenphysiol. 106:97 (1982).Google Scholar
- 13.P. C. Bilkey and E. C. Cocking, Isolation and properties of plant microplasts: newly identified subcellular units capable of wall synthesis and division into separate micro cells, Eur. J. Cell Biol. 22:502 (1980).Google Scholar
- 17.D. Pental, S. Cooper-Bland, K. Harding, E. C. Cocking, and A. J. Müller, Cultural studies on nitrate reductase deficient Nicotiana tabacum mutant protoplasts, Z. für Pflanzenphysiol. 105:219 (1982).Google Scholar
- 18.J. Draper, M. R. Davey, J. P. Freeman, E. C. Cocking, and B. J. Cox, Ti-plasmid homologous sequences present in tissues from Agrobacterium plasmid-transformed Petunia protoplasts, Plant Cell Physiol. 23:92 (1982).Google Scholar