Gene Organization and Regulation in Neurospora Crassa. Evidence from the Cloning and Transformation of the qa Gene Cluster

  • Mary E. Case
Part of the NATO Advanced Science Institutes Series book series (NSSA, volume 61)


An analysis of the qa. gene cluster in Neurospora offers an excellent opportunity for understanding the organization and regulation of a eukaryotic gene cluster. This cluster is involved in the catabolism of quinic acid and encodes three closely linked structural genes, qa-2, qa-3, and qa-4, and a positively acting regulatory gene, qa-1, Fig. 1 (Giles et al., 1978). The (qa gene cluster is very tightly linked to me-7 on the right arm of LG VII and so far has proved to be inseparable from the centromere. The qa-2 gene encodes catabolic dehydroquinase; the qa-3 gene, quinate dehydrogenase; and the qa-4 gene, 5-dehydroshikimate dehydratase, Fig. 1. The qa-2 gene was cloned by complementation of an aroD - strain of E. coli which lacks activity for the biosynthetic dehydroquinase (Vapnek et al., 1977). The entire qa gene cluster has been cloned on a 19 kb fragment utilizing the cosmid technique (Collins and Hohn, 1980) and selecting for qa-2 + complementation in E. coli (Schweizer et al., 1981). In E. coli, only the qa-2 + gene is expressed. The qa-3 + , qa-4 + and qa-1 + genes were detected on the basis of transformation back into Neurospora with these cosmid clones.


Gene Cluster Neurospora Crassa Quinic Acid Southern Hybridization Analysis Replacement Type 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Alton, N.K., Buxton, F., Patel, V., Giles, N.H., and Vapnek, D., 1982, 5′ untranslated sequences of two structural genes in the qa gene cluster of Neurospora crassa, Proc. Natl. Acad. Sci., 79:1955–1959.PubMedCrossRefGoogle Scholar
  2. Case, M.E., 1982, Transformation of Neurospora crassa, in “Genetic Engineering of Microorganisms,” A. Hollaender, R.D. DeMoss, S. Kaplan, J. Konisky, D. Savage, and R.S. Wolfe, ed., Plenum Publishing Corp., New York.Google Scholar
  3. Case, M.E. and Giles, N.H., 1975, Genetic evidence on the organization and action of the qa-1 gene product: a protein regulating the induction of three enzymes in quinate catabolism in Neurospora crassa, Proc. Natl. Acad. Sci., USA, 72:553–557.PubMedCrossRefGoogle Scholar
  4. Case, M.E., Hautalia, J.A., and Giles, N.H., 1977, Characterization of qa-2 mutants in Neurospora crassa by genetics, enzymatic, and immunological techniques, Bacteriol. 129:166–172.Google Scholar
  5. Case, M.E., Schweizer, M., Kushner, S.R. and Giles, N.H., 1979, Efficient transformation of Neurospora crassa utilizing hybrid Plasmid DNA, Proc. Natl. Acad. Sci., USA, 76:5259–5263.PubMedCrossRefGoogle Scholar
  6. Giles, N.H., Alton, N.K., Case, M.E., Hautala, J.A., Jacobson, J.W., Kushner, S.R., Patel, V.B., Reinert, W.R., Strøman, P., and Vapnek, D., 1978, The organization of the qa gene cluster in Neurospora crassa and its expression in Escherichia coli, 1978 Stadler Genetics Symposia, 10:47–63.Google Scholar
  7. Hicks, J.B., Hinnen, A., and Fink, G.R., 1978, Properties of yeast transformation, Cold Spring Harbor Symp., Vol. XLIII, 1305–1313.Google Scholar
  8. Hinnen, A., Hicks, J.B., and Fink, G.R., 1978, Transformation of yeast, Proc. Natl. Acad. Sci., USA, 75:1929–1933.PubMedCrossRefGoogle Scholar
  9. Kingsman, A.J., Gimlich, R.L., Clarke, L. , Chinault, A.C., and Carbon, J. , 1981, Sequence variation in dispersed repetitive sequences in Saccharomyces cerevisiae, J. Mol. Bio. 145:619–632.CrossRefGoogle Scholar
  10. Patel, V.B., Schweizer, M., Dykstra, C.C., Kushner, S.R., and Giles, N.H., 1981, Genetic organization and transcriptional regula­tion in the qa gene cluster of Neurospora crassa, Proc. Natl. Acad. Sci., 78:5783–5787.PubMedCrossRefGoogle Scholar
  11. Rine, J., Jensen, R., Hagen, D., Blair, L. and Herskowitz, I., 1980, Pattern of switching and fate of the replaced cassette in yeast mating-type interconversion, Cold Spring Harbor Symposium, 78: 951–960, Vol. 2.Google Scholar
  12. Schweizer, M., Case, M.E., Dykstra, C.C., Giles, N.H. and Kushner, S.R., 1981, Identification and characterization of recombinant plasmids carrying the complete qa gene cluster from Neurospora crassa including the qa-1 +regulatory gene, Proc. Natl. Acad. Sci., 78:5086–5090.PubMedCrossRefGoogle Scholar
  13. Szabo, G. and Schablik, M., 1982, Behavior of DNA-induced inositol-independent transformants of Neurospora crassa in sexual crosses, Theor. Appl. Genet., 61:171–175.CrossRefGoogle Scholar
  14. Vapnek, D., Hautala, J.A., Jacobson, J.W., Giles, N.H., and Kushner, S.R., 1977, Expression in Escherichia coli K-12 of the structural gene for catabolic dehydroquinase of Neurospora crassa, Proc. Natl. Acad. Sci., 74:3508–3512.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1983

Authors and Affiliations

  • Mary E. Case
    • 1
  1. 1.Department of Molecular and Population GeneticsUniversity of GeorgiaAthensUSA

Personalised recommendations