Liposomes as a Tool for Introducing Biologically Active Viral Nucleic Acids into Plant Protoplasts
Lipid vesicles (liposomes) have successfully been used to introduce a variety of biological macromolecules into mammalian cells. In particular large unilamellar vesicles prepared with the Ca++ -EDTA chelation technique (LUV) have been used for delivering infective poliovirus particles(1) and RNA(2) into permissive (HeLa) and non permissive (CHO,L) cells. More recently Fraley et al.(3) have reported the encapsulation of simian virus 40(SV40) DNA in large unilamellar vesicles prepared with the reverse-phase evaporation technique (4) and its delivery in an infectious form to monkey cells in culture.
KeywordsTobacco Mosaic Virus Leaf Mesophyll Plant Protoplast Tobacco Protoplast Large Unilamellar Vesicle
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