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Liposomes as a Tool for Introducing Biologically Active Viral Nucleic Acids into Plant Protoplasts

  • Franco Rollo
Part of the NATO Advanced Science Institutes Series book series (NSSA, volume 61)

Abstract

Lipid vesicles (liposomes) have successfully been used to introduce a variety of biological macromolecules into mammalian cells. In particular large unilamellar vesicles prepared with the Ca++ -EDTA chelation technique (LUV) have been used for delivering infective poliovirus particles(1) and RNA(2) into permissive (HeLa) and non permissive (CHO,L) cells. More recently Fraley et al.(3) have reported the encapsulation of simian virus 40(SV40) DNA in large unilamellar vesicles prepared with the reverse-phase evaporation technique (4) and its delivery in an infectious form to monkey cells in culture.

Keywords

Tobacco Mosaic Virus Leaf Mesophyll Plant Protoplast Tobacco Protoplast Large Unilamellar Vesicle 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1983

Authors and Affiliations

  • Franco Rollo
    • 1
  1. 1.Istituto di Microbiologia e Fisiologia VegetaleUniversita di PaviaPaviaItaly

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