Reversal of Plant Tumor Formation

  • Milton P. Gordon
Part of the NATO Advanced Science Institutes Series book series (NSSA, volume 61)


The transformation of plant tissues by the soil bacteria Agrobacterium tumefaciens and Agrobacterium rhizogenes results in tumor formation or a callus with excessive root formation, respectively. These processes are associated with the incorporation of bacterial plasmid DNA (T-DNA), into the genome of the host plant. Much effort is underway to utilize these processes to incorporate useful genes into plants by using the bacterial plasmid as a transformation vector. In order for the processes to be useful, it is necessary to have a procedure whereby normal fertile plants can be generated from the tumor tissue with retention of the foreign genes.


Hairy Root Crown Gall Agrobacterium Rhizogenes Bacterial Plasmid Crown Gall Tumor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Braun, A.C. (1959). A demonstration of the recovery of the crown-gall tumor cell with the use of complex tumors of single cell origin. Proc. Natl. Acad. Sci. U.S.A. 45:932–938.PubMedCrossRefGoogle Scholar
  2. 2.
    Braun, A.C. and Wood, H.N. (1976). Suppression of the neoplastic state with the acquisition of specialized functions in cells, tissues, and organs of crown gall teratoma of tobacco. Proc. Natl. Acad. Sci. U.S.A. 73:496–500.PubMedCrossRefGoogle Scholar
  3. 3.
    Chilton, M.D., Tepfer, D.A., Petit, A., David, C, Casse-Delbart, F., and Tempe, J. (1982). Agrobacterium rhizogenes inserts T-DNA into the genomes of the host plant root cells. Nature, 295:432–34.CrossRefGoogle Scholar
  4. 4.
    Leemans, J. Deblaere, P.. Willmitzer, L., De Greve, H., Hernal-steens, J.P., Van Montagu, M. and Schell, J. (1982). The Embo Journal. 1(1):147–152.PubMedGoogle Scholar
  5. 5.
    Lutz, A. (1966). Obtention de plantes de tabac a partir de cul-tures unicellulaires provenant d’une souche anergée. CR. Acad. Sci. Paris. 262:1856–58.Google Scholar
  6. 6.
    Ooms, G., Bakker, A., Molendijk, L., Wullems, G., Gordon, M.P., Nester, E.W., and Schilperoort, R..A., (1982). T-DNA organization in homogeneous and heterogeneous octopine-type crown gall tissues of Nicotiana tabacum. Cell (in press).Google Scholar
  7. 7.
    Otten, L., De Greve, H., Hernalsteens, J.P., Van Montagu, M., Scheider, O., Straub, J., and Schell, J. (1981). Mol. Gen. Genet. 183:209–213.PubMedCrossRefGoogle Scholar
  8. 8.
    Powell, Ann T., Unpublished.Google Scholar
  9. 9.
    Ream, W., Unpublished.Google Scholar
  10. 10.
    Sacristan, M.D., and Melchers, G. (1969). The caryological analysis of plant regeneration from tumorous and other callus cultures of tobacco. Mol. Gen. Genet. 105:317–333.PubMedCrossRefGoogle Scholar
  11. 11.
    Sacristan, M.D. and Melchers, G. (1977). Regeneration of plants from habituated and agrobacterium-transformed single-cell clones of tobacco. Mol. Gen. Genet. 152:111–117.Google Scholar
  12. 12.
    White, F., Ghidossi, G., Gordon, M.P., and Nester, E.W. (1982). Tumor induction of Agrobacterium rhizogenes involves the transfer of plasmid DNA to the plant genome. Proc. Natl. Acad. Sci. U.S.A. 79:3193–97.PubMedCrossRefGoogle Scholar
  13. 13.
    Wullems, G.J., Molendijk, L., Ooms, G., and Schilperoort, R.A. (1981). Retention of tumor markers in F1 progeny plants from in vitro induced octopine and nopaline tumor tissues. Cell. 24:719–727.PubMedCrossRefGoogle Scholar
  14. 14.
    Yang, F.-M., and Simpson, R. (1981). Revertant seedlings from crown gall tumors retain a portion of the bacterial T-DNA sequences. Proc. Natl. Acad. Sci. U.S.A. 78:4151–4155.PubMedCrossRefGoogle Scholar
  15. 15.
    Yang, F.-M., Merlo, D.J., Drummond, M.H., Chilton, M.-D., Nester, E.W., and Gordon, M.P. (1980a). Plasmid DNA of Agro-bacterium tumefaciens detected in a presumed habituated tobacco cell line. Mol. Gen. Genet. 177:704–714.CrossRefGoogle Scholar
  16. 16.
    Yang, F.-M., Montoya, A., Merlo, D.J., Drummond, M.H., Chilton, M.-D., Nester, E.W., and Gordon, M.P. (1980b). Foreign DNA sequences in crown gall teratomas and their fate during the loss of the tumorous traits. Mol. Gen. Genet. 177:707–714.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1983

Authors and Affiliations

  • Milton P. Gordon
    • 1
  1. 1.Department of BiochemistryUniversity of WashingtonSeattleUSA

Personalised recommendations