Construction of Recombinant Ti Plasmids Containing the chlM Gene from E. Coli

  • Thianda Manzara
  • Paul F. Lurquin
Part of the NATO Advanced Science Institutes Series book series (NSSA, volume 61)


The stable maintenance of foreign genes in plant cells is dependent upon the nature of the vector used in DNA transfer attempts. The potential vectors which presently exist can be divided into two categories: 1) vectors which integrate within the host genome and 2) vectors which can be established as independent replicons in plant cells. The first category includes the T-DNA from Agrobac-terium tumefaciens and the T-DNA from Agrobacterium rhizogenes. The second class of vectors comprises the caulimovirus and germinivirus genomes, as well as cDNA copies of viroids. The pros and cons of each of those vectors have been recently reviewed by Howell1.


Nitrate Reductase Activity Crown Gall Sole Nitrogen Source Agrobacterium Rhizogenes Marker Exchange 
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Copyright information

© Plenum Press, New York 1983

Authors and Affiliations

  • Thianda Manzara
    • 1
  • Paul F. Lurquin
    • 1
  1. 1.Program in Genetics and Cell BiologyWashington State UniversityPullmanUSA

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