Construction of Cloning Vectors from the IncW Plasmid pSa and their use in Analysis of Crown Gall Tumor Formation
A set of four cloning vectors has been constructed from the wide host range IncW plasmid pSa. Although the vectors are transfer defective, three of them can be efficiently transferred into a wide variety of Gram-negative bacteria by a separate mobilizing plasmid. One of the vectors is a cosmid and can be used for cloning large DNA fragments. The vectors, which can be selected on the basis of kanamycin, spectinomycin, or chloramphenicol resistance, contain cloning sites for seven different restriction endonucleases. DNA fragments from the A. tumefaciens plasmid TiC58 have been cloned into these vectors, and used to complement avirulent TiC58::Tn5 mutants.
KeywordsCloning Vector Crown Gall Conjugal Transfer KpnI Site Insertional Inactivation
Unable to display preview. Download preview PDF.
- 12.R. C. Tait, T. J. Close, R. L. Rodriquez, and C. I. Kado, Gene, in press (1982).Google Scholar