Bacterial-Plant Gene Cloning Shuttle Vectors for Genetic Modification of Plants
Directed genetic transformation of plant cells is one of the fundamental requirements for tailoring plants’ cells to harbor desirable characteristics. This requirement can be achieved by the development of systems for the delivery and integration of foreign genes into the plant genome. Two potential gene delivery systems have been the focus of considerable attention: the Ti plasmid of Agrobacterium tumefaciens and the DNA plant virion cauliflower mosaic virus (CaMV)1–8. Although these are the most thoroughly characterized systems, reports on the successful application of these vectors in the manipulation of the plant genome have been premature. The inherent limitations of these vectors have been previously pointed out3,4,6,7, yet the design of a cloning vector capable of replication in bacteria and plants continues to center around the Ti plasmid and DNA plant viruses.
KeywordsCloning Vector Shuttle Vector Crown Gall KpnI Site SaIl Site
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- 1.P. J. J. Hooykaas, R. A. Schilperoort, and A. Rorsch, Genetic Engineering Vol, 1, p. 151–179, J. K. Setlow and A. Hollaender eds., Plenum Press, New York-London (1973).Google Scholar
- 3.C. I. Kado, and A. Kleinhofs, Intl. Rev. Cytology, Suppl. IIB, 47–80, I. K. Vasil, ed., Academic Press, New York (1980).Google Scholar
- 5.R. B. Meagher, and T. D. McKnight, Genome Organization and Expression in Plants, C. J. Leaver, ed., p. 63–75. Plenum Press, New York-London (1980).Google Scholar
- 8.J. Schell, and M. Van Montagu, Transfer of cell constituents into Eukaryotic cells, J. E. Cells, ed., p. 325–346. Plenum Press, New York-London (1980).Google Scholar
- 9.C. I. Kado, R. C. Tait, R. C. Lundquist, M. Hagiya, D. Zaitlin, and D. Gallie, Proc. Fourth Intl. Symp. Genetics Indust. Microorg., Kyoto, Japan (In Press) (1982).Google Scholar
- 10.R. C. Tait, T. J. Close, R. C. Lundquist, M. Hagiya, R. L. Rodriguez, and C. I. Kado, Proc. Natl. Acad. Sci. USA (submitted) (1982).Google Scholar
- 18.J. Schell, M. Van Montagu, M. Holsters, J. P. Hernalsteens, H. DeGreve, J. Leemans, L. Willmitzer, L. Otten, J. Schroder, and G. Schroder, Fourth Int. Symp. Genetic Industr. Microorg. Kyoto, Japan (Abstract) (1982).Google Scholar