Bacterial-Plant Gene Cloning Shuttle Vectors for Genetic Modification of Plants

  • C. I. Kado
  • R. C. Tait
Part of the NATO Advanced Science Institutes Series book series (NSSA, volume 61)


Directed genetic transformation of plant cells is one of the fundamental requirements for tailoring plants’ cells to harbor desirable characteristics. This requirement can be achieved by the development of systems for the delivery and integration of foreign genes into the plant genome. Two potential gene delivery systems have been the focus of considerable attention: the Ti plasmid of Agrobacterium tumefaciens and the DNA plant virion cauliflower mosaic virus (CaMV)1–8. Although these are the most thoroughly characterized systems, reports on the successful application of these vectors in the manipulation of the plant genome have been premature. The inherent limitations of these vectors have been previously pointed out3,4,6,7, yet the design of a cloning vector capable of replication in bacteria and plants continues to center around the Ti plasmid and DNA plant viruses.


Cloning Vector Shuttle Vector Crown Gall KpnI Site SaIl Site 
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Copyright information

© Plenum Press, New York 1983

Authors and Affiliations

  • C. I. Kado
    • 1
  • R. C. Tait
    • 1
  1. 1.Department of Plant PathologyUniversity of CaliforniaDavisUSA

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