Laser Doppler Microscopy: Especially as a Method for Studying Brownian Motion and Flow in the Sieve Tubes of Plants

  • Richard P. C. Johnson
Part of the NATO Advanced Science Institutes Series book series (NSSA, volume 59)


Laser Doppler velocimetry and photon correlation spectroscopy are well established as methods for measuring the flow and diffusion of particles in fluids. The diameters of the laser beams used are usually too wide to be placed precisely within plant or animal cells, capillaries, or other specimens of interest to biologists. Several groups of workers have described laser Doppler microscopes, either with crossed beams or with single beams. These instruments allow scattered laser light to be detected from volumes with diameters of 10 pm or less and enable the scattering volume to be seen and placed accurately in specimens viewed at high magnification. Some designs and principles of laser Doppler microscopes are discussed, especially of a single beam instrument and its application to the study of Brownian motion and flow in sieve tubes, the food transport channels in higher plants.


Brownian Motion Autocorrelation Function Objective Lens Interference Fringe Laser Doppler Velocimetry 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Plenum Press, New York 1983

Authors and Affiliations

  • Richard P. C. Johnson
    • 1
  1. 1.The Botany DepartmentUniversity of AberdeenOld AberdeenScotland

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