Laser Light Scattering Study of the Fractionation of Casein Micelles in Skim Milk by Controlled Pore Glass Chromatography

  • M. C. A. Griffin
  • M. Anderson
Part of the NATO Advanced Science Institutes Series book series (NSSA, volume 59)


Casein is a major protein component of milk. It consists of several different polypeptides: αS1, αS2, β and к casein, as well as varying amounts of proteolytic fragments of these polypeptides. The polypeptides are bound together by calcium phosphate and citrate to form aggregates of a wide range of sizes (Holt et al, 1978) up to 680 nm in diameter (McGann et al, 1980). The size distribution of these casein aggregates, known as ‘micelles’, in milk may change with different conditions such as variation in Ca2+ concentration, pH and history of heat treatment. The nature of the size distribution may affect functional properties of the milk such as enzymic coagulation on addition of rennet (Ekstrand et al, 1980; Dalgleish et al, 1981). It has proved desirable to characterize the size distribution of casein micelles in milk so as to investigate related properties further.


Photon Correlation Spectroscopy Calcium Phosphate Skim Milk Hydrodynamic Diameter Casein Micelle 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Brehm, G.A. & Bloomfield, V.A. (1975) Macromolecules 8: 663–5.ADSCrossRefGoogle Scholar
  2. Brown, J.C. and Pusey, P.N. (1974) J. Phys. D 7; L31 - L35.ADSCrossRefGoogle Scholar
  3. Brown, J.C., Pusey, P.N. and Dietz, R. (1975) J. Chem. Phys. 62: 1136–1144.ADSCrossRefGoogle Scholar
  4. Dalgleish, D.G., Brinkhuis, J. and Payens, T.A.J. (1981) Eur. J. Biochem. 119; 257–261.Google Scholar
  5. Ekstrand, B., Larsson-Raznikiewicz, M. and Perlmann, C. (1980) Biochim. Biophys. Acta 630; 361–366.CrossRefGoogle Scholar
  6. Holt, C., Kimber, A.M., Brooker, B. & Prentice, J.H. (1978) J. Coli. Int. Sci. 65; 555–565.CrossRefGoogle Scholar
  7. Jenness, R. and Koops, J. (1962) Neth. Milk and Dairy J. 16: 153–164.Google Scholar
  8. Larsson-Raznikiewicz, M., Almlöf, E. and Ekstrand, B. (1979) J. Dairy Res. 46: 313–316.CrossRefGoogle Scholar
  9. McGann, T.C.A., Donnelly, W.J., Kearney, R.D. & Buchheim, W. (1980) Biochim. Biophys. Acta 630: 261–270.CrossRefGoogle Scholar
  10. The Coulter Nano-Sizer’ by the Fine Particle Group, Coulter Electronics Ltd, Luton, UK. January 1980 pp. 10.Google Scholar

Copyright information

© Plenum Press, New York 1983

Authors and Affiliations

  • M. C. A. Griffin
    • 1
  • M. Anderson
    • 1
  1. 1.Physical Sciences DepartmentNational Institute for Research in DairyingShinfield, Reading, BerkshireEngland

Personalised recommendations