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Studies of Neurotransmitter Receptor Interactions Using Quantitative Video Intensification Microscopy (V.I.M.)

  • D. G. MacInnes
  • D. K. Green
  • A. Harmar
  • E. G. Nairn
  • G. Fink
Part of the NATO Advanced Science Institutes Series book series (NSSA, volume 59)

Abstract

Studies of interactions between neurotransmitters and their receptors would be greatly facilitated by a method to obtain cell fractions enriched with cells that contain a high density of receptors specific for the neurotransmitter. Here we report the use of a fluorescence activated cell sorter to prepare fractions of pituitary cells that contain a high density of high affinity, specific receptors for the decapeptide, luteinizing hormone releasing hormone (LHRH). Since no enzymatic procedures are employed, the dispersed cells permit both binding and biological responses to be analysed immediately without requiring the usual time of 16–48hr for recovery.

Keywords

Fluorescence Activate Cell Sorter Pituitary Cell Fluoresce Cell Luteinizing Hormone Release Hormone High Performance Liquid Chromato 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Fink, G. 1979 Neuroendocrine control of gonadotrophin secretion. British Medical Bulletin, 35: 2, 155–160Google Scholar
  2. Green, D.K., Malloy, P., Steel, M. 1980. The recovery of living cells by flow sorting machine. Flow Cytometry IV; 103–106.Google Scholar
  3. Hazum, E., Cuatrecasas, P., Marian, J., Conn, P.M. 1980. Receptor—mediated internalization of fluorescent gonadotropinreleasing hormone by pituitary gonadotropes. Proc. Natl. Acad. Sci., U.S.A. 77: 6692–6695CrossRefGoogle Scholar
  4. Speight, A., Fink, G. 1981. Changes in responsiveness of dispersed pituitary cells to luteinizing hormone releasing hormone at different times of the oestrous cycle of the rat. J. Endocr. 89: 129–134.Google Scholar

Copyright information

© Plenum Press, New York 1983

Authors and Affiliations

  • D. G. MacInnes
    • 1
  • D. K. Green
    • 2
  • A. Harmar
    • 1
  • E. G. Nairn
    • 1
  • G. Fink
    • 1
  1. 1.MRC Brain Metabolism UnitUniversity Department of PharmacologyEdinburghUK
  2. 2.MRC Clinical and Population Cytogenetics UnitWestern General HospitalEdinburghUK

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