A Note on Taking Aliquots from Small Volumes of Upper Phases

  • H. de Bree
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 12)


In the development of a drug assay method we ran into difficulties connected with the use of small volumes. It appeared to be feasible to extract the analyte completely from 2 ml of aqueous solution into 50 µl of isooctane; but to take an aliquot of the organic layer into a syringe for chromatography was troublesome. It was hardly possible to sample the organic phase without contaminating it with the aqueous phase.


Aqueous Solution Layer Thickness Aqueous Phase Organic Phase Small Volume 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Copyright information

© Plenum Press, New York 1983

Authors and Affiliations

  • H. de Bree
    • 1
  1. 1.Duphar Research LaboratoriesWeespThe Netherlands

Personalised recommendations