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Quantitative HPTLC in Assaying Body Fluids for Drugs and Metabolites

  • W. Ritter
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 12)

Abstract

High-performance thin-layer chromatography (HPTLC) is an instrumentalized approach, more reliable than conventional TLC, and superior in other respects such as sensitivity. Commercially available instrumentation, e.g. for plate development in its different modes, is considered, and the optimization of a spotting device (seeAppendix); Examples of applications are given later (art. #D-5).

Keywords

Derivatization Reaction Densitometric Measurement Microlitre Syringe Solvent Surface Tension Twin Trough Chamber 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

References

  1. 1.
    Zlatkis, A. & Kaiser, R.E. (eds.) (1977) HPTLC High Performance Thin-Layer Chromatography, Elsevier, Amsterdam, and Institute of Chromatography, Bad Dürkheim, 240 pp.Google Scholar
  2. 2.
    Bertsch, W., Hara, S., Kaiser, R.E. & Zlatkis, A. (eds.) (1980) Instrumental TLC, Hüthig, Heidelberg.Google Scholar
  3. 3.
    Fenimore, D.C., Meyer, C.J. & Davis, C.M. (1977)J. Chromatog. 1U2, 399–409Google Scholar
  4. 4.
    Ritter, W. (1977)J. Chromatog. 142, 431–440.CrossRefGoogle Scholar
  5. 5.
    Fenimore, D.C. & Meyer, C.J. (1979)J. Chromatog. 186, 555–561CrossRefGoogle Scholar
  6. 6.
    Touchstone, J.C. & Levin, S.L. (1980)J. Liq. Chromatog. 3, 1853–1863.CrossRefGoogle Scholar
  7. 7.
    Pohl, U. & Schweden, W. (1975)Z. Anal. Chem. 274, 265–269.CrossRefGoogle Scholar
  8. 8.
    Faber, D.B., de Kok, A. & Brinkman, U.A. Th. (1977)J. Chromatog. 143, 95–103.CrossRefGoogle Scholar
  9. 9.
    Sondergaard, I. & Steinness, E. (1979)J. Chromatog. 162, 422–426.CrossRefGoogle Scholar
  10. 10.
    Schäfer. M., Geissler, H.E. & Mutschier, E. (1977)J. Chromatog. 143, 615–623.Google Scholar
  11. 11.
    Steyn, J.M. (1977)J. Chromatog. 143, 210–213-Google Scholar
  12. 12.
    Lieber, E.R. & Taylor, S.L. (1978)J. Chromatog. 160, 227–237.CrossRefGoogle Scholar
  13. 13.
    Ritter, W., Plempel, M. & Pütter, J. (1974)Arzneim.-Forsch. Drug Res J24, 521–525Google Scholar
  14. 14.
    Bratton, A.C. & Marshall, E.K. (1939)J. Biol. Chem. 128, 537–550.Google Scholar
  15. 15.
    Haefelfinger, P. (1978)J. Chromatog. 145, 445–451.CrossRefGoogle Scholar
  16. 16.
    Faber, D.B., Mulder, C. & Man in’t Veld, W.A. (1974)J Chromatog. 100, 55–61.CrossRefGoogle Scholar
  17. 17.
    Huizing, G., Beckett, A.H. & Segura, J. (1979)J. Chromatog. 172, 227–237.CrossRefGoogle Scholar
  18. 18.
    Haefelfinger, P. (1976)J. Chromatog. 12k, 351–358.CrossRefGoogle Scholar
  19. 19.
    Gupta, R.N., Eng, F. & Keane, P.M. (1977)J. Chromatog. 143, 112–114.CrossRefGoogle Scholar
  20. 20.
    Steiness, I., Christiansen, J. & Steiness, E. (1979)J. Chromatog. 164, 241–246.CrossRefGoogle Scholar

Additional References

  1. 9.
    Kirchner, J.G. (1973)J. Chromatog. 82, 431–440.Google Scholar
  2. 10.
    DeAngelis, R.L., Robinson, M.M. & Sigel, C.W. (1980)J. Liq. Chromatog. 3, 833–839.CrossRefGoogle Scholar
  3. 11.
    Fenimore, D.C. (1980) in Instrumental HPTLC (Bertsch, W., Hara, S., Kaiser, R.E. & Zlatkis, A., eds.) , Huthig, Heidelberg, pp. 81–95Google Scholar

Copyright information

© Plenum Press, New York 1983

Authors and Affiliations

  • W. Ritter
    • 1
  1. 1.Department of PharmacokineticsBayer AGWest Germany

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