Problems Arising from Assaying Low Concentrations by HPLC

  • C. R. Jones
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 12)


Assays of drugs and metabolites by HPLC are frequently carried out at very high sensitivity, i.e. conditions of low signal-to-noise ratio. This brings in train a number of problems; some are well known, others are not suspected by those workers who deal only with samples at high concentration. The examples given represent problems that have occurred in a drug metabolism bioanalytical laboratory. Readers may add to the list.


Check Valve Baseline Drift Large Injection Volume Soda Glass Fluorescein Solution 


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    Scales, B. (1981) as for 1., pp. 353–363.Google Scholar
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    Feyerabend, C. & Russell, M.A.H. (1980)J. Pharm. Pharmacol. 32, 178–181.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1983

Authors and Affiliations

  • C. R. Jones
    • 1
  1. 1.Wellcome Research LaboratoriesBeckenham, KentUK

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