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Determination of 3-Phenoxy-N-Methylmorphinan and Metabolites in Plasma

  • J. A. F. de Silva
  • J. Pao
  • M. A. Brooks
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 12)

Abstract

The drug (I, Fig. 1) is one of the phenoxymorphinan analogues synthesized by Mohacsi [1] and is under development as a non-narcotic analgesic. It is extensively metabolized in the rat [2] and in the dog [3] byN-demethylation to form the nor-analogue (I-A),and byp-hydroxylation of the 3-phenoxy ring to yieldI-Band cleavage of the ether linkage to yield the morphinanI-Cand itsN-demethylation produces nor-levorphanol (I-D) (Fig. 1). The presence of four metabolites besides the drug necessitated a strategy for the development of sensitive and specific assays for their quantitation. Of the approaches tried, with dog plasma during pre-clinical drug development, GC-MS (+ve CI) proved the most satisfactory.

Keywords

Ether Linkage 111M Phosphate Buffer Dichloromethane Medium Chemical Research Division Peak Monitor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Plenum Press, New York 1983

Authors and Affiliations

  • J. A. F. de Silva
    • 1
  • J. Pao
    • 1
  • M. A. Brooks
    • 1
  1. 1.Department of Pharmacokinetics and BiopharmaceuticsHoffmann-La Roche Inc.NutleyUSA

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