Immunoassay of Bromocriptine and Specificity of Antibody: Criteria for Choice of Antiserum and Marker Compound
To develop an assay for bromocriptine (2-bromo- α-ergokryptine), different hapten compounds were used to produce various animal antisera. Since all these were predominantly specific to the tricyclic peptide moiety of bromocriptine, the affinity constant Kwas considered a sufficient criterion for selecting a high energy antibody. The K value for the favoured antiserum (#270979) was 0.9 x 10 9 1/M. With the favoured tracer ligand, a detection limit of 0.17 ng/ml was achieved for bromocriptine in equilibrium with its epimer bromocriptinine. Metabolites such as the 8′-hydroxy derivative show virtually no cross-reaction.
KeywordsErgot Alkaloid Lysergic Acid Methane Sulphonate Tracer Compound High Specific Radioactivity
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