Quantification of Metabolites of Aminopyrine and Antipyrine in Plasma and Urine
Both aminopyrine and antipyrine serve as test compounds for assessing hepatic microsomal activityin vivo[ 1 , 2]. Several published assays [3–6] allow their plasma concentrations to be measured over a time period of 4–5 times their half-life. However, the usefulness of the two drugs would be much increased if routine analytical methods were available to quantify their metabolites and hence allow pharmacokinetic studies on the individual metabolic pathways rather than on the gross behaviour of the parent drug.
KeywordsHydrolysis HPLC Chloroform Acetonitrile Chloro
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