HPLC Analysis of the Major Urinary Metabolite of Flurazepam

  • J. A. F. de Silva
  • M. A. Brooks
  • M. R. Hackman
  • R. E. Weinfeld
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 12)


Pharmacokinetic parameters of a drug are commonly evaluated by the determination of unchanged drug in blood or plasma. An acceptable alternative [1], especially if a non-invasive method is desired, is to use urinary excretion data for either the unchanged drug or a major metabolite. If the latter represents a significant percentage of the administered dose, its quantitation places fewer constraints on the sensitivity required of the analytical procedure, and on the sample preparation and ‘clean-up’ required prior to analysis. HPLC, either normal-phase (NP) or reverse-phase (RP), is ideally suited to this type of determination [2]. RP conditions have now been developed.


Unchanged Drug Control Urine Differential Pulse Polarography Urinary Excretion Data Major Urinary Metabolite 
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  1. 1.
    Federal Register (1977) 42 (5) , 1624–1653Google Scholar
  2. 2.
    de Silva, J.A.F. (1981) in Trace-Organic Sample Handling (Vol. 11, this series; Reid, E., ed.) , Horwood, Chichester, pp. 192–204.Google Scholar
  3. 3.
    Schwartz, M.A. & Postma, E. (1970)J. Pharm. Sci. 59, 1800–1806.CrossRefGoogle Scholar
  4. 4.
    de Silva, J.A.F. & Strojny, N. (1971)J. Pharm. Sci. 60, 1303–1314.CrossRefGoogle Scholar
  5. 5.
    de Silva, J.A.F., Puglisi, C.V., Brooks, M.A. & Hackman, M.R. (1974)J. Chromatog. 99, 461–483.CrossRefGoogle Scholar
  6. 6.
    Peat, M.A. & Kopjak, L. (1979)J. Forens Sci. 24, 46–54.Google Scholar
  7. 7.
    de Silva, J.A.F., Bekersky, I. & Puglisi, C.V. (1974) J. Pharm.Sci. 63, 1837–1841.Google Scholar
  8. 8.
    Glover, W., Earley, J., Delaney, M. Dixon, R. (1980)J. Pharm. Sci. 69, 601–602.CrossRefGoogle Scholar
  9. 9.
    Clatworthy, A.J., Jones, L.V. & Whitehouse, M.J. (1977)Biomed Mass Spectrometry4, 248–254CrossRefGoogle Scholar
  10. 10.
    Miwa, B.J., Garland, W.A. & Blumenthal, P. (1981)Anal. Chem. 54, 793–797CrossRefGoogle Scholar
  11. 11.
    Weinfeld, R.E. & Miller, K.F. (1981)J. Chromatog. 223, 123–130.CrossRefGoogle Scholar
  12. 12.
    Brooks, M.A. (1980) in Electroanalysis in Hygiene, Environmental, Clinical and Pharmaceutical Chemistry (Franklin-Smyth, W., ed.) , Elsevier, Amsterdam, pp. 287–298.Google Scholar
  13. 13.
    Kackman, M.R. & Brooks, M.A. (1981)J. Chromatog. 222, 179–190.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1983

Authors and Affiliations

  • J. A. F. de Silva
    • 1
  • M. A. Brooks
    • 1
  • M. R. Hackman
    • 1
  • R. E. Weinfeld
    • 1
  1. 1.Department of Pharmacokinetics and BiopharmaceuticsHoffmann-La Roche Inc.NutleyUSA

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