Chromatography, Hydrolysis and Rearrangement of the Glucuronide Conjugate of Isoxepac, as Affecting its Assay in Urine

  • I. D. Wilson
  • A. Bhatti
  • H. P. A. Illing
  • T. A. Bryce
  • J. Chamberlain
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 12)

Abstract

In man the non-steroidal anti-inflammatory drug isoxepac is excreted into the urine, mainly as an ester glucuronide. A pH-dependent conversion of the presumed 1-0-acyl glucuronide into several enzyme-resistant conjugates is observed. All the rearrangement products may he hydrolyzed, using mild alkali, to isoxepac. This conversion intoβ-glucuronidase-resistant conjugates has a bearing on the assay of isoxepac and its glu-uronides, and of other drugs excreted as ester glucuronides. Suitable procedures for the determination of total isoxepac in human urine are described.

Keywords

Human Urine Free Drug Alkaline Hydrolysis Glucuronide Conjugate Clofibric Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1983

Authors and Affiliations

  • I. D. Wilson
    • 1
  • A. Bhatti
    • 1
  • H. P. A. Illing
    • 1
  • T. A. Bryce
    • 1
  • J. Chamberlain
    • 1
  1. 1.Hoechst Pharmaceutical Research LaboratoriesMilton Keynes, Bucks.UK

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