Chromatography, Hydrolysis and Rearrangement of the Glucuronide Conjugate of Isoxepac, as Affecting its Assay in Urine
In man the non-steroidal anti-inflammatory drug isoxepac is excreted into the urine, mainly as an ester glucuronide. A pH-dependent conversion of the presumed 1-0-acyl glucuronide into several enzyme-resistant conjugates is observed. All the rearrangement products may he hydrolyzed, using mild alkali, to isoxepac. This conversion intoβ-glucuronidase-resistant conjugates has a bearing on the assay of isoxepac and its glu-uronides, and of other drugs excreted as ester glucuronides. Suitable procedures for the determination of total isoxepac in human urine are described.
KeywordsHuman Urine Free Drug Alkaline Hydrolysis Glucuronide Conjugate Clofibric Acid
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- 1.Illing, H.P.A. & Fromson, J.M. (1978)Drug. Metab. Dispos. 6, 510–517.Google Scholar
- 2.Levy, G.A. (1952)Biochem. J. 52, 464–472.Google Scholar
- 7.Blanckaert, N. Compernolle, F., Leroy, P., van Houtte, R., Fevery, J. & Heirwegh, K.P.H. (1978)Biochem. J. 171, 203–214.Google Scholar
- 9.Sinclair, K.A. k Caldwell,J. (1981)Biochem. Soc. Trans. 9, 215.Google Scholar
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- 11.Illing, H.P.A. & Wilson, I.D. (1981) Biochem. Pharmacol. 30, 3381–3384.Google Scholar