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Isolation and Characterization of Amino Acid and Sugar Conjugates of Xenobiotic Carboxylic Acids

  • John Caldwell
  • Andrew J. Hutt
  • Mary Varwell Marsh
  • Keith A. Sinclair
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 12)

Abstract

The carboxyl group in xenobiotics such as certain drugs and pesticides may be conjugated with glucuronic acid, glycine, glutamine or taurine. A logical system, developed for excreta but of general applicability, is now presented for isolating and characterizing such conjugates. The choice of TLC or HPLC system is determined by the mobility of the parent acid. It cannot be taken for granted that all alkali-labile conjugates are ester glucuronides. Problems in splitting off and identifying different conjugate moieties are considered. Chemical synthesis of amino acid conjugates is facile and aids identification.

Keywords

Nuclear Magnetic Resonance Spectrum Glucuronic Acid Clofibric Acid Mixed Anhydride Intramolecular Rearrangement 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1983

Authors and Affiliations

  • John Caldwell
    • 1
  • Andrew J. Hutt
    • 1
  • Mary Varwell Marsh
    • 1
  • Keith A. Sinclair
    • 1
  1. 1.Department of PharmacologySt. Mary’s Hospital Medical SchoolLondonUK

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