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Preparative Liquid-Chromatographic Methods in Drug Metabolism Studies

  • W. Dieterle
  • J. W. Faigle
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 12)

Abstract

The main difficulties in isolating drug metabolites from biological materials lie in their low concentrations in urine, bile or blood, and in the high content of endogenous compounds and food constituents. The physico-chemical properties of the more strongly polar metabolites further complicate their isolation in pure form. Preparative liquid chromatography ( ‘prep-LC’ ) offers many advantages. As is now exemplified, it enables the products to be isolated in quantities sufficient for full structure elucidation by spectroscopic and chemical methods. It can be applied to complex mixtures of metabolites of widely varying polarities. Since separation is done at low temperature, normally no stability problems arise. Recoveries can easily be checked if radioactively labelled drugs are used.

Keywords

Packing Material Polar Metabolite Drug Metabolite Individual Metabolite Crude Material 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1983

Authors and Affiliations

  • W. Dieterle
    • 1
  • J. W. Faigle
    • 1
  1. 1.Research Department, Pharmaceuticals DivisionCIBA-GEIGY LimitedBasleSwitzerland

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