Examples of Approaches to Isolating and Identifying Phase I Metabolites

  • G. R. Bourne
  • S. R. Moss
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 12)


The Phase I metabolites of ICI 89,406, a β-adrenoreceptor blocking drug, were isolated from bile from the perfused rat liver preparation, and the structures determined by NMR spectroscopy. The metabolic fate of the veterinary product cloprostenol (‘Estrumate’*), a prostaglandin analogue, was studied in vivo in the target species, the cow. GC-MS was used to identify drug-derived urinary components that had been isolated by a range of chromatographic procedures. In the study of cloprostenol metabolism the high isotopic abundance of14C in radio-labelled cloprostenol allowed an unusual method of detecting metabolites.


Prostaglandin Analogue Hydroxyl Function Imperial Chemical Industry Veterinary Product Unusual Method 
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  1. 1.
    Bourne, G.R., Moss, S.R., Phillips, P.J. & Shuker, B. (1980)Biomed. Mass Spec. 7, 226–230.CrossRefGoogle Scholar
  2. 2.
    Bourne, G.R., Moss, S.R., Phillips, P.J., Webster, J.T.A. & White, D.F. (1979)Biomed. Mass Spec. 6, 359–360.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1983

Authors and Affiliations

  • G. R. Bourne
    • 1
  • S. R. Moss
    • 1
  1. 1.Drug Kinetics Goup, Safety of Medicines Department, Pharmaceuticals DivisionImperial Chemical Industries PLCAlderley Park, near MacclesfieldUK

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