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Examples of Approaches to Isolating and Identifying Phase I Metabolites

  • G. R. Bourne
  • S. R. Moss
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 12)

Abstract

The Phase I metabolites of ICI 89,406, a β-adrenoreceptor blocking drug, were isolated from bile from the perfused rat liver preparation, and the structures determined by NMR spectroscopy. The metabolic fate of the veterinary product cloprostenol (‘Estrumate’*), a prostaglandin analogue, was studied in vivo in the target species, the cow. GC-MS was used to identify drug-derived urinary components that had been isolated by a range of chromatographic procedures. In the study of cloprostenol metabolism the high isotopic abundance of14C in radio-labelled cloprostenol allowed an unusual method of detecting metabolites.

Keywords

Prostaglandin Analogue Hydroxyl Function Imperial Chemical Industry Veterinary Product Unusual Method 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Bourne, G.R., Moss, S.R., Phillips, P.J. & Shuker, B. (1980)Biomed. Mass Spec. 7, 226–230.CrossRefGoogle Scholar
  2. 2.
    Bourne, G.R., Moss, S.R., Phillips, P.J., Webster, J.T.A. & White, D.F. (1979)Biomed. Mass Spec. 6, 359–360.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1983

Authors and Affiliations

  • G. R. Bourne
    • 1
  • S. R. Moss
    • 1
  1. 1.Drug Kinetics Goup, Safety of Medicines Department, Pharmaceuticals DivisionImperial Chemical Industries PLCAlderley Park, near MacclesfieldUK

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