Examples of Approaches to Isolating and Identifying Phase I Metabolites
The Phase I metabolites of ICI 89,406, a β-adrenoreceptor blocking drug, were isolated from bile from the perfused rat liver preparation, and the structures determined by NMR spectroscopy. The metabolic fate of the veterinary product cloprostenol (‘Estrumate’*), a prostaglandin analogue, was studied in vivo in the target species, the cow. GC-MS was used to identify drug-derived urinary components that had been isolated by a range of chromatographic procedures. In the study of cloprostenol metabolism the high isotopic abundance of14C in radio-labelled cloprostenol allowed an unusual method of detecting metabolites.
KeywordsProstaglandin Analogue Hydroxyl Function Imperial Chemical Industry Veterinary Product Unusual Method
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