A Note on Quantitation of14C Radioactivity in HPLC Eluates

  • D. W. Roberts
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 12)


The main advantage associated with the use of radiolabelled compounds in HPLC, as extensively practised in our Section’s studies of potential drugs, is that a simple non-specific method of detection applies to all labelled components derived from the drug without interference from sample-associated impurities.


Liquid Scintillation Counting Fraction Time Counting Efficiency Confidence Range Fraction Collection 


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Relevant literature

  1. 1.
    Mackey, L.N., Rodriguez, P.A. & Schroeder, F.B. (1981)J. Chromatog. 208, 1–8.CrossRefGoogle Scholar
  2. 2.
    Reeve, D.R., Yokota, T., Nash, L.S. & Crozier, A. (1976)J. Exp. Bot. 27, 1243–1258.CrossRefGoogle Scholar
  3. 3.
    Webster, H.K. & Whaun, J.M. (1981)J. Chromatog. 209, 283–292.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1983

Authors and Affiliations

  • D. W. Roberts
    • 1
  1. 1.Drug Metabolism Section Safety of Medicines Department ICI Pharmaceuticals DivisionMacclesfieldUK

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