A Note on Rapid Removal of Fine Particles from Alumina for Column Chromatography

  • B. L. Goodwin
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 12)


The background to the procedure shown in Plate 1 is that alumina is an essential tool in the measurement of catecholamines and analogues since they are fairly specifically adsorbed at slightly alkaline pH and are readily eluted at acidic pH. After the requisite acid pre-wash, alumina is a powder with a large range of particle sizes. It is essential to remove the finer particles, since they drastically reduce the flow rate through an alumina column. The traditional way [1] entailing washing with water is inefficient as it removes little more than colloidal material, and is very-time consuming.


Column Chromatography Large Range Fine Particle Rapid Method Alumina Particle 
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  1. 1.
    Weil-Malherbe, H. (1961) in Methods in Medical Research, Vol. 9 (Quastel, J.H., ed.) , Year Book Medical Publishers, Chicago, pp. 130–146.Google Scholar

Copyright information

© Plenum Press, New York 1983

Authors and Affiliations

  • B. L. Goodwin
    • 1
  1. 1.Bernhard Baron Memorial Research LaboratoriesQueen Charlotte’s HospitalLondonUK

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