Strategies Centered on HPLC
The choice of an HPLC system will depend on its application and on the nature of the metabolites to be measured. Normal-phase (NP) chromatography is limited to the measurement of drugs whose physico-chemical properties are similar to the parent drug, whereas reverse-phase (RP) chromatography in its various forms has made possible the simultaneous measurement of metabolites varying widely in pK a , lipophilicity and polarity. Either alone or in combination with other chromatographic techniques, HPLC has aided the isolation of some polar metabolites which may be difficult to isolate from biological material. In contrast with other types of chromatography, thein vitroformation of metabolites can be monitored directly by HPLC, with minimal sample preparation. Thus HPLC will aid the study of enzyme kinetics and reaction mechanisms.
KeywordsRetention Index Polar Metabolite Clofibric Acid Sulphate Conjugate Minimal Sample Preparation
Unable to display preview. Download preview PDF.
Chapter 1 References
- 2.Several authors (1980)J. Chromatog. Sci. 18, 393–486.Google Scholar
- 3.Several authors (1980)J. Chromatog. Sci. 18, 487–582.Google Scholar
- 4.Allan, R.J., Goodman, H.T. & Watson, T.R. (1980) J. Chromatog. 183, 311–319.Google Scholar
- 12.Ruelius, H.W., Tio, C.O., Knowles, J.A., McHugh, S.L., Schillings, R.T. & Sisenwine, S.F. (1979)Drug Metab. Disp. 7, 40–43Google Scholar